|
Objective To investigate the ulinastatin ( ulinastatin ) on rat hind limb ischemia - reperfusion liver protective effect and the possible mechanism . To establish a healthy SD rat hind limb ischemia - reperfusion model , 24 healthy male SD rats were randomly divided into three groups ( n = 8 ) . Sham operation group only underwent anesthesia, heparin, the right hind relaxation surrounded by a rubber band , do not block the blood flow , I operate with ischemic - reperfusion group , blood and liver tissue equipment seized . The control group underwent anesthesia, heparin, surrounded by a rubber band ligation of the right hind limb roots, blocking blood flow release after 4 h recovery after 4 h of perfusion , blood and liver tissue equipment seized . Experimental group with the model before ischemia immediately dorsal penile vein be Ulinastatin 5 × 104 U / kg , the meter 0.5 ml , sham-operated group and the control group received normal saline intravenously . Liver function tests , serum TNF - a , IL - 10 factor concentration , electron microscopy of liver mitochondrial structure and optical microscopy structure of liver pathology . Results of liver function parameters were measured in the control group , the experimental group compared with the sham group, ALT , AST , LDH increased in the experimental group compared with the control group ALT , AST , LDH reduce inflammatory factors TNF - a , IL - 10 was measured in the control group , the experimental group than in the sham-operated group elevated TNF-a in the experimental group compared with the control group, TNF - a reduce , control group , the experimental group compared with help from an array of IL-10 increased in the experimental group than the control group increased IL-10 , with significant difference ( P lt ; 0.05 ) . Mitochondrial structure: the Prosthetic Hand array : 0 , control group ( n = 2-3 ) , one of the experimental group . Conclusion This study table Ming Wu ulinastatin has a significant protective effect of lower limb ischemia - reperfusion injury induced liver damage , and its mechanism may be reduced pro - inflammatory factors TNF - a to enhance the inhibition of inflammatory cytokines IL - 10 to reduce the inflammation, protect the liver cells , reduce liver damage caused by lower limb ischemia - reperfusion injury .
|