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The method for determination of phthalic esters from Plastic bottled liquor by HPLC isresearched,using n-hexane as liquid-liquid extraction solvent,water bath heating time2min,Then it measured by HPLC, using external standard method to quantitative. In the rangeof0.5～100mg/L,it has the good linear relationship (R2>0.9998);the lowest detectable limitsare0.015～0.047mg/L;Standard solution as the precision test,relative standard deviation in0.03%～0.40%,showed that the precision of this method is good.The recovery rate of thephthalic esters at10mg/L,20mg/L and50mg/L,standard addition recovery rate was between80.2%and97.7%,the relatively standard deviation is in1.76%～2.74%,less than5%,and themethod has a high accuracy.Gas chromatography-mass spectrometer for the determination of phthalate esters in Plasticbottled liquor has been developed.Within the scope of0.5～100mg/L presents a good linearrelationship (R2>0.9998).The method detection limits of DEP and DNOP were0.05mg/L,whilethose of DBP and DEHP were0.005mg/L;To blank wine add the PAEs mixed standard solutionconcentration was5mg/L and50mg/L respectively to precision test,the relation standarddeviation ranged from1.57%to3.53%,which indicated the method’s precision to meet therequirements for the determination of PAEs in liquor. On the add level of20mg/L,50mg/L,80mg/L,the recovery rate of PAEs is in the ranged of87.85%～97.38%,the relatives standarddeviation is in1.10%～4.10%.which are within5%. The method is simple,rapid,sensitive,accurate,suitable for detection of PAEs in plastic bottled liquor in qualitative and quantitative.By employing the single factor experiment combined with response surface methodology,the relationship of extraction time, the ratio of material to solvent, extraction power andextraction volume was founded,the optimal levels were as follows: the extraction solvents isacetone, the extraction temperature of50℃, the extraction time of31min,the ratio of material tosolvent is1:42, the extraction power of90W,the PAEs extracting amount was46.21mg/kg,which was higher than before. Provided the basic data for food safety.By using distilled water,3％a cetic,10％eth anol,65％ethanol and isooctane as five kinds offood simulant.Distilled water,3％a cetic,10％e thanol,65％ethanol test samples were extractedwith trichlormethane.Under different conditions to separation and extraction of PAEs of foodsimulation,the results show that migration amount of DBP and DEHP increases withtemperature rising and time prolonging,migration amount of PAEs in food simulants arearranged in the following order: isooctane>65％ethanol>10％e thanol>3％acetic>distilledwater,including DEHP than DBP migration trend is obvious.10%ethanol and65%ethanolwere compared,65%ethanol is more than PAEs migration volume of10%ethanol. Actual sample migration experiment,under the20℃low-alcohol wine28.4degrees,theStrong wine58degrees,35.5degrees plum wine.The migration rate of PAEs from threedifferent alcohol content of wine was studied at4℃,25℃and37℃by HPLC.The resultsshow that PAEs migration increase gradually with the increase of temperature,.At4℃and25℃, however,failed to detect DNOP in low-alcohol wine and plum wine;at37℃,the migrationamount of PAEs more than the former temperature,migration amount of PAEs inorder:DEHP>DBP>DEP>DNOP,failed to detect DNOP in low-alcohol wine.Comparison ofstrong wine,low-alcohol wine and plum wine,three of the PAEs migration quantity is alwaysstrong wine> plum wine>low-alcohol wine. The result shows that it was consistent with themigration trend of ethanol at different concentrations in food simulants.
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