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The Investigation of Corelation between PRDM1 and Rb and Their Impact on Cell Cycle Control

Author: ZhangZuo
Tutor: YuLi；WangLiLi
School: Nankai University
Course: Clinical
Keywords: PRDM1 Rb Cell cycle G1 phase arrest Hela cells
CLC: R730.2
Type: Master's thesis
Year: 2010
Downloads: 65
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Abstract

Objective: PRDM1 a, β , and Rb three internal relations and their different functions in cell cycle regulation , further reveal the the PRDM1 protein and Rb interaction of the cell cycle . METHODS: The experiment is divided into four parts . The first part will be specific for the Rb gene of SiRNA (SiRb) , transfected into Hela cells , a transient knockdown cell model of the Rb protein , transfection the Hela cells unrelated SiRNA (SiNS) as a negative control . The second part , respectively, in knockout and not knock addition to the expression of Rb in Hela cells transfected PRDM1 a vector or empty vector negative control cells by flow cytometry analysis after PI staining , observed and compared before and after removal in Rb the PRDM1 a cell cycle regulation . The third part , respectively, in the knockout and not knock addition to Rb Hela cells transfected PRDM1β expression vector or negative control empty vector cells by flow cytometry after PI staining analysis of observed and compared before and after the removal in Rb PRDM1β impact on the regulation of the cell cycle . Part IV , respectively, in the Rb hela cells grow well into the plasmid containing the empty vector , to containing the PRDM1 a cDNA plasmid containing PRDM1βcDNA of the plasmid by western blot protein electrophoresis detection, observation of Rb protein expression status and the degree of phosphorylation . Results: 1. Hela cell line expression of Rb PRDM1a can the majority of cells in G1 phase arrest . In the Rb expression hela cell lines PRDM1a lift G1 cell cycle arrest function . Before and after 2.Rb knockout , PRDM1β expression in Hela cells are not led to the arrest of the G1 phase of the cell cycle . Overexpressed in Hela cells 3. PRDM1a part of the Rb protein can be maintained at the non-phosphorylated or activated state the while PRDM1β does not have this effect . Conclusion : PRDMla can induce some tumor cells arrest in the G1 phase of the cell cycle , and this effect was dependent on the presence of Rb protein , and one of the molecular basis of the mechanism of action is part of the Rb protein can be maintained at the non-phosphorylated or activated state because PRDM1a . The PRDM1β not having the above-described functions . The results of this study the prompt PRDM1a , and PRDM1β have different biological functions , PRDMla may have the role of the tumor suppressor gene , the while PRDM1β may be related to tumor progression .

The Investigation of Corelation between PRDM1 and Rb and Their Impact on Cell Cycle Control

Abstract

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