Dissertation > Excellent graduate degree dissertation topics show

Enrichment Culture of Mixed Methanotrophic Community and Its Application in Coal Mine Gas Control

Author: JiangZuo
Tutor: XingXinHui
School: Tsinghua University
Course: Chemical Engineering and Technology
Keywords: methanotrophs MMO molecular ecology fermentation coal gas control
CLC: X172
Type: PhD thesis
Year: 2010
Downloads: 130
Quote: 0
Read: Download Dissertation


Methanotrophs have great potential for applications in CH4 removal and gas control of coal mines. But the methanotrophs that can be used in complex environment are limited due to low growth rate and unstable MMO activity. Also, there is no substantial applied research on gas control by methantophs.In this study, the methanotroph community of the soil from a typical coal mine in China was analyzed by multiple molecular ecology techniques to estabilish the strategies of methanotrophic enrichment cultures. Two mixed methanotrophic communities were enriched by different domesticating conditions. The characteristics and capabilities of the two methanotrophic communities were evaluated, and large-scale fementation was studied. Lab and field pilot experiments were designed to explore the feasibility of using the mixed methanotrophic community for CH4 removal and gas control.Several complementary molecular ecology techniques were used to elucidate the methanotrophic community from a coal mine. There were lots of methanotrophs and methylotrophs found in the coal mine soils, in which typeⅠwas the main part. The ratios of the typeⅠ, typeⅡand typeⅩmethanotrophs were 78.6%, 7.1% and 14.3%.CH4 and artificial mash gas were used to domesticate mixed methanotrophic cultures capable of stably and effectively abating CH4, respectively. The capability of CH4 and C2H6 removal was enhanced apparently during the enrichment. The diversity of the total bacteria community decreased, and the amount of the typeⅠmethanotrophs increased obviously. After 20 generations, the microbial community and CH4 removal ability became stable.The characteristics of the two mixed methanotrophic communities enriched above, including morphology, growth, metabolism and microbial community structure, were then studied. The amount of the typeⅡmethanotrophs increased in both cultures, and there were more kinds of bacteria in the community enriched with the artificial mash gas. The CH4 removal abilities of the two mixed methanotrophic communities were stronger than the pure methanotroph. There were no metabolites accumulated during the cultivation. The synergistic effects of the different microorganisms were beneficial for maintaining the stability of the microbial community and the capability of avoiding product inhibition.The mixed methanotrophic communities showed good CH4 removal performance under different CH4 concentrations (350%) and different temperatures (2042oC). And they were tolerant to the toxic gases consisting of H2S, CO and SO2 which always exist in the coal mine gas. The mixed methanotrophic communities were well preserved by three methods: 4oC sealing with CH4, -80oC freezing collected cells, and -80oC freezing with paraffin.The scale-up fermentation for a mixed methanotrophic community was realized from 5L to 300L and from 5L to 600L systems, respectively. The mixed methanotrophic culture grew quickly in large-scale fermentation. The CH4 removal ability and microbial community were kept stable during the different scale fermentation.In the lab-scale experiments of simulate coal bed and biofitration, the performance of CH4 removal by the mixed methnaotrophic community was investigated. And then, pilot-scale study for gas control in coal beds was carried out in three coal mines in Henan. The prepared culture of the mixed methanotrophic community was injected into the coal bed, and the CH4 was removed effectively.

Related Dissertations

  1. Haihong Fruit Vinegar Processing Technology,TS264.22
  2. Research on Anaerobic Fermentation Processing Technology of Dead Tilapia,TS254.4
  3. Characterization and Identification of Maturity of Pig Manure Composting,S141.4
  4. Effects of 2-Hydroxy-4-Methionine Isopropyl on Rumen Fermentation and Production Performance of Holstein Cows,S823.8
  5. Study on the Breeding of High Efficient Detoxification Strains and Suggestion Parameters of Solid Fermentation on Cottonseed Meal,S816.6
  6. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  7. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  8. Insecticidal Activities of Recombinant CryIAc Protein from Bacillus Thuringiensis and Solid Fermentation Products from Aspergillus Terreus PNR2,S476.1
  9. The Study of Adding Wet Brewer’s Grains on Fermentation Quality of Mixed Ensilages of Corn Stalk and Agricultural Byproducts,S816.53
  10. Study on Mixed Strain for Liquid State Fermentation of Animal Blood,S816.4
  11. Investigation on the Diversity and Phylogenetic of Methanogens and the Relationship between Methanogenesis and Acetogenesis in the Hindgut of Pigs,S828
  12. Studies on the Extraction of Total Flavonoids from Momordica Charantia L. and the Production of Alcohol by By-product,TQ914.1
  13. Research on the Mutation Breeding of Flavomycin and Regulation of Fermentation Techniques,TQ920.6
  14. Study on Radix Pueraria Isoflavones Extraction by Fermentation and Its Antioxidation,TQ920.6
  15. Studys of the Protein Extract and Utilization of Producing Protein Feed from Tea Residues,S816
  16. The Mutation Screening and the Production Optimization of Avermectin Producing Strain,TQ450.6
  17. Pyrohydrolysis of Corn Stover Catalyzed by Dilute Nitric Acid and Biological Acidification of Hydrolysate,X712
  18. Design and Analysis of a High-Efficiency Biogas Production System with Solar Heating Based on Thermal Management Controller,TK51
  19. Design on Anaerobic Co-Digester with Reflux Stirring and Study on Fermentation Process,S216.4
  20. Study on Influential Factor to Methane Metabolic of Methane-utilizing Bacteria and Methoanol Detection Method,Q93
  21. Study of Solid Liquid Mixed Bacterial Fermentation of Water Hyacinth Production of Cell Protein Feed,S816

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
© 2012 www.DissertationTopic.Net  Mobile