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[Objective] To explore different points in time given erythropoietin (EPO) on pentylenetetrazol (PTZ) the epileptic rat pups epilepsy (EP) after the onset of cerebral protection. [] Clean grade inbred 60 SD male 4-5 week-old rat pups using random number divided into six groups: the experimental group (AE, 50) intraperitoneal injection of PTZ 50 mg / (kg.) and control group (F, n = 10) by intraperitoneal injection of an equal volume of saline. Is further divided into experimental group the simple epileptic group (A, 10) and EPO intervention group (BE Group), in seizures before 24h (group B, 10), after the onset of 0h (group C, 10), 6h (D, n = 10), 9h (E, 10), respectively, intraperitoneal injection of rhEPO 5000u / (kg.); each group in the EP after 24h take hippocampus, enzyme-linked immunosorbent assay in neuron-specific ene alcohol enzyme (NSE), myelin basic protein (MBP) and S100beta level; each group were randomly selected two young rats hippocampal tissue by immunohistochemistry was used to observe morphological changes EPO epileptic young brain damage protection case. [Results] 1. Behavior performance the the AE rats are typical epilepsy behavioral changes, the F group pups behavior did not change. 24 hours ago rhEPO intervention group seizures the pups seizures extent and severity than that induced epilepsy group significantly reduced, P lt; 0.01. 2. Changes in hippocampus NSE level hippocampal concentrations of NSE in Group A, E group were significantly increased, and B group, C group D group, F group is statistically significant, P lt; 0.05; hippocampus NSE concentration in B group, C group D group F group was no significant difference, P gt; 0.05 hippocampus. MBP levels in the hippocampus MBP concentration in Group A, E group were significantly increased, and B group, C group D group, F group is statistically significant, P lt; 0.05; B group, C group D group F hippocampus MBP concentration difference between the two groups no significant resistance, P gt; 0.05. 4 hippocampus S100beta concentration, changes in the hippocampus S100beta protein level in Group A, E group were significantly increased, with group B, group C, group D, group F comparison with statistically significant, P lt; 0.05; B group, C group D group F group hippocampus S100beta concentration difference was not significant, P gt; 0.05 hippocampus NSE, S100beta MBP protein immunohistochemical staining Group F number of positive cells can be seen LT; 25%, the cytoplasm light brown yellow. A group of 75% -100% positive cells, stained dark brown brown. BD group was 25% -50% positive cells stained light brown yellow to brown. Group E 50% -75% positive cells staining deepen brownish yellow to brown color. 6 serum endogenous EPO detection of serum endogenous EPO levels the difference was not statistically significant. [Conclusions] (1) rhEPO may have antiepileptic effects. (2) rhEPO PTZ immature epileptic rats induced with neuroprotective effects, especially in the EP after 6h administration role.
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