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Objective To investigate the effect of hydroxyethyl starch(HES 130/0.4) on the expression of aquaporin 1(AQP-1) and liver injury in rats with severe acute pancreatitis(SAP) Methods Forty-eight male SD rats were randomly divided into Sham group,SAP group and HES group;each group were divided into 6 hour and 24 hour,with 8 each point. SAP model was induced by injecting 5% sodium taurodeoxycholate into biliary pancreatic duct. AMY,ALT,AST and water content in the liver were measured and TNF-a was examined by ELISA, and the expression of liver AQP-1mRNA was determined by RT-PCR,and the expression of liver AQP-1 protein was evaluated by immunohistochemical methods. Results Compared with Sham group, the level of AMY,ALT,AST , TNF-a,water content, AQP-1mRNA and AQP-1 protein increased significantly in SAP group (P<0.05) while Compared with SAP group, the level of AMY,ALT,AST ,TNF-a,water content, AQP-1mRNA and AQP-1 protein decreased significantly in HES group (P<0.05).At 6th hour, the expression of liver AQP-1mRNA were(0.402±0.023)、(0.811±0.032)and(0.595±0.015)in Sham、SAP and HES group respectively;At 24th hour, they were(0.412±0.017)、(0.823±0.029)and(0.607±0.021),with significant difference each group (P<0.05)。At 6th hour, the expression of liver AQP-1 proteins were(2.07±0.25)、(6.90±0.38)and(4.48±0.29)in Sham、SAP and HES group respectively;At 24th hour, they were(2.32±0.31)、(7.04±0.32)and(4.56±0.35) , with significant difference each group (P<0.05)。Compared with SAP group,the pathology of pancreas Compared with SAP group,the pathology of pancreas and liver ameliorated significantly in HES group. Conclutions hydroxyethyl starch 130/0.4 may ameliorate the liver injury of severe acute pancreatitis,and alleviate the capillary leak. AQP-1 may play a role in the capillary leak caused by the liver injury of severe acute pancreatitis.
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