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The purpose of detection of systemic lupus erythematosus (SLE) and normal control group blood leukocyte expression levels of Annexin Ⅱ and plasma t-PA levels compared to patients with chronic glomerulonephritis (CGN), diabetic nephropathy (DN), explore the Annexin Ⅱ in systemic lupus erythematosus significance. The collected First Affiliated Hospital of Suzhou University hospital from January 2009 to October 2009 by the Department of Nephrology, 35 cases of patients with systemic lupus erythematosus (in line with the 1997 American College of Rheumatology classification criteria for SLE diagnosis), 10 cases of patients with chronic nephritis, diabetic nephropathy 10 patients with normal, healthy control group of 20 patients. Annexin Ⅱ expression levels of white blood cells by flow cytometry; plasma levels of t-PA by enzyme-linked immunosorbent assay (ELISA) detection; the detection blood pressure to extract the fasting blood detection hemoglobin content, serum creatinine, blood cholesterol, blood sugar and thrombin indicators of the original time, partial thromboplastin time, D-dimer; determined by radioimmunoassay urinary albumin. Results 1 healthy control group comparison, in patients with systemic lupus erythematosus group blood leukocytes Annexin Ⅱ level significantly lower (P lt; 0.01), plasma t-PA level significantly higher (P lt; 0.01); diabetic nephropathy group patients blood leukocytes Annexin Ⅱ level also significant with lower (P lt; 0.05), plasma t-PA level difference (P gt; 0.05); chronic nephritis group of patients with blood leukocyte Annexin II and plasma t-PA level had no significant forward difference (P gt; 0.05) . Compared with systemic lupus erythematosus, diabetic nephropathy group of white blood cells in patients with the level of Annexin Ⅱ no significant sex difference (P gt; 0.05), plasma t-PA levels were significantly lower (P lt; 0.01). Systemic lupus erythematosus patients with lupus nephritis patients blood leukocyte Annexin Ⅱ levels than those without nephritis patients decreased, but there was no significant difference (P gt; 0.05), plasma t-PA levels, there was no significant differences (P gt; 0.05). Part of the patients with lupus nephritis renal biopsy Ⅳ lupus nephritis patients with plasma levels of t-PA, the comparison of the different types of pathology, the most obvious, the of Annexin Ⅱ levels of white blood cells in no significant differences among pathological types. Grouped according to the disease activity index comparison: comparison with inactive (SLEDAI score lt; 9 points) in patients with systemic lupus erythematosus, patients with white blood cell activity (SLEDAI score ≥ 9 points) Annexin Ⅱ levels were significantly lower (P lt; 0.05 ), t-PA had no significant difference (P gt; 0.05). 4 compared with the healthy control group, patients with systemic lupus erythematosus group of D-dimer levels were significantly higher (P lt; 0.01), prothrombin time, partial thromboplastin time and fibrinogen showed no significant difference ( P gt; 0.05). Correlation analysis, Annexin Ⅱ level and urinary protein / creatinine showed a negative correlation (r = -0.382, p lt; 0.05), and albumin were positively correlated (r = 0.439, p lt; 0.01), SLEDAI was negatively correlated ( r = -0.417, p lt; 0.05), and D-dimer were negatively correlated (r = -0.336, p lt; 0.05), and 24-hour urine protein (r = -0.319, p gt; 0.05), blood total cholesterol (r = -0.230, p gt; 0.05), hemoglobin (r = -0.005, p gt; 0.05), serum creatinine (r = -0.204, p gt; 0.05) correlation was significant. t-PA level and blood sugar showed a positive correlation (r = 0.602, p-lt; 0.01), and erythrocyte sedimentation rate was positive related (r = 0.53, p-lt; 0.01), IgG was positive related (r = 0.468, p-lt; 0.01). Plasma t-PA levels and leukocyte Annexin Ⅱ no significant difference (r = -0.014, P GT; 0.05). The Conclusion in patients with systemic lupus erythematosus exist the hypercoagulable and secondary hyperfibrinolysis state of coagulation and fibrinolytic system abnormalities involved in the pathophysiological process of the development of systemic lupus erythematosus. 2 Annexin Ⅱ decrease may be one of the important mechanisms that lead to a hypercoagulable state of patients with systemic lupus erythematosus and prone to thrombosis. 3 Annexin Ⅱ as a reflection of the body's blood clotting and fibrinolytic system abnormalities new mark, can serve as a good indicator of the Early reflect systemic lupus erythematosus prothrombotic state, some help judge lupus activity and efficacy.
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