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The Research of Transplanting the Human Embryonic Germ Cells to Treat the Acute Myocardial Infarction of the Pig

Author: ChenQing
Tutor: ChenYongZhen
School: Suzhou University
Course: Human Anatomy and Embryology
Keywords: Human embryonic germ cells Cell transplantation VEGF GATA-4 Cx43
CLC: R542.22
Type: Master's thesis
Year: 2010
Downloads: 14
Quote: 0
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Abstract


Objective: to establish the model of acute myocardial infarction ; 2 . Observe human embryonic germ cells (human embryonic germ cells, hEGCs) ported to swine acute myocardial infarction model of myocardial survival and differentiation ; 3 . Explore human EG cell transplantation in myocardial tissue expression and significance of vascular endothelial growth factor (vascular endothelial growth factor, VEGF) . Methods: 5-10 weeks abortion human embryonic gonadal ridges cultured human EG cells using tissue culture method . Human EG cells cultured alone , while EG cells co-cultured embryonic cardiomyocytes to simulate myocardial microenvironment , both 3 days and 9 days culture supernatant of VEGF expression levels were detected . By ligation of the left anterior descending artery was established swine model of acute myocardial infarction (AMI) model is divided into transplantation group and control group . Transplant group in vitro expansion of human EG cells were injected into swine infarcted myocardium surrounding the control group injected with sterile PBS in the same way . Were sacrificed 2 weeks after transplantation, pig , mouse anti-human cell nucleus the antibody MAB1281 as a tracer detection survival of transplanted cells by immunohistochemistry and the to observe transcription factor GATA -4 Expression of Cx43 in the expression of the transplanted cells . Results: In vitro cultivation to obtain a large number of human EG cells , human EG cells and embryonic myocardial cells co-cultured group three days and nine days the supernatant VEGF levels were higher than EG cells cultured alone group , the difference was significant significance ( P lt ; 0.05 ) ; successful swine acute myocardial infarction model , typical seen after ligation of the coronary ECG changes in myocardial infarction ; immunohistochemistry showed anti-human transplantation group the nucleus antibody MAB1281, transcription factor GATA-4, and connexin Cx43 were expressed in control group as a negative expression . Conclusion: establishing epicardial transplantation of human EG cells in acute myocardial infarction model , human EG cells in the host myocardium survival and differentiation into myocardial cells gradually , implanted human EG cells may promote the infarcted area through the secretion of cytokines such as VEGF cardiac repair .

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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases > Myocardial infarction
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