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Isolation, Purification and Structure Characterization and Biological Activity of the Polysaccharides from Pleurotus Tuber-regium(Fr.)Sing Sclerotium

Author: ZhangPeng
Tutor: ZhangJing
School: Shaanxi Normal University
Course: Of Food Science
Keywords: Pleurotus tuber-regium sclerotia alkaline polysaccharides Antioxidant chemical structure Molecular conformation
CLC: S567.39
Type: Master's thesis
Year: 2013
Downloads: 25
Quote: 0
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Pleurotus tuber-regium(Fr.)Sing Sclerotium is the very edible and medicinal fungi,belonging to Basidiomycotina.Pleurotus tuber-regium(Fr.)Sing Sclerotium polysaccharides contain various biological activities,such as antioxidant.anti-aging, enhance immunity。At present, there are more research reports about the cultivation conditions of Pleurotus tuber-regium, the chemical structure is unclear, in this paper, the alkli-soluble polysaccharides was obtained after extracted, separated and purificated, from Pleurotus tuber-regium(Fr.)Sing Sclerotium。The antioxidant, chemical structure, solution behavior of Pleurotus tuber-regium(Fr.)Sing Sclerotium polysaccharides were studied.1.extraction, separation and purification of APPSlThe hot-alkli extraction PPS was adopted to extract polysaccharides from Pleurotus tuber-regium(Fr.)Sing Sclerotium, After alcohol precipitation,dialylsis,concentration and freeze drying, the crude extraction was obtained and further fractionated on DEAE-52and Sepharose G-150column chromatography, giving one fraction termed APPSl. Ultraviolet spectrophotometry and HPLC analysis results showed that the APPSl was an homogeneous ingredients with an average molecular weight5.96×105Da.2.Antioxidant activity in vitro of the polysaccharides from Pleurotus tuber-regium(Fr.)Sing Sclerotium,The polysaccharides from Pleurotus tuber-regium(Fr.)Sing Sclerotium were determined using the total reducing capacity, ability of hydroxyl radical scavenging in vitro, superoxide anion radical scavenging ability, scavenging ability of DPPH·.The experimental results show that three kinds of polysaccharides had some the antioxidant capacity. There was dose-effect relationship with the concentration of antioxidant polysaccharides。Found in the determination of scavenging hydroxyl free radical and superoxide anion free radical ability.PPS showed the strongest scavenging effect, there may be various components in the crude polysaccharides。The coordination effect of several components is stronger than a component。In the removal of DPPH·experiment, APPS1had the strongest ability, This showed that this component may be the active component of scavenging DPPH· crude polysaccharide in Pleurotus tuber-regium sclerotia alkali extraction。3.The chemical structure of the APPS1HPLC,GC and IR were used to analyze the structure of APPSl,and that there was only glucose in the APPSl,and the unit was β-D-pyran-glucose.Through Periodate oxidation, Smith degradation, there were (1→) linked,(1→6) linked,(1→2) linked and (1→4) linked、(1→3) linked,(1→2) linked,(1→linked,(1→6) linked,(1→2,6) linked.4.solution behavior of Pleurotus tuber-regium(Fr.)Sing Sclerotium polysaccharidesFrom the pH, temperature, denaturation, factors of polysaccharide concentration and metal ions on Pleurotus tuber-regium polysaccharide APPS1viscosity was studied。 Each of the factors on the effect of viscosity ratio of each are not identical.Congo red experiment showed that the Pleurotus tuber-regium(Fr.)Sing Sclerotium polysaccharide APPS1with helical structure.

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