Dissertation > Excellent graduate degree dissertation topics show

Isolation and Expression Analysis of ZFP Genes from Eucalyptus grandis and Screening of Transcription Factors from the Promoter of EgrZFP1

Author: WangShuai
Tutor: TongZaiKang
School: Zhejiang Forestry University
Course: Tree Genetics and Breeding
Keywords: Eucalyptus grandis C2H2-type zinc finger gene gene cloning expression yeast one-hybrid
CLC: S792.39
Type: Master's thesis
Year: 2013
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


C2H2-type zinc finger proteins are a major family of transcriptional factors and play a crucial role innot only plant growth and development but also plant response to stresses. In plants, the first C2H2-typezinc finger gene was isolated in Xenopus tropicalis, named ZPT2-1, and genes of this type zinc fingerprotein have gradually been isolated from Arabidopsis thaliana, cotton, soybeans, rice and other plants.Lots of them have been identified in herbaceous plants but few were clarified in woody plants.Seven genes with two zinc finger motifs were isolated from Eucalyptus grandis by homologous,named as EgrZFP1-7. They all belonged to Q-type C2H2zinc finger gene family and were classifiedinto subclass C1, containing a QALGGH motif. Except EgrZFP2, all of them contain an EAR motif.Expression pattern of EgrZFP1-7genes showed that no obvious expression change was found inleaves, stems and roots except EgrZFP4, which had higher transcripts level in roots than in leaves andstems and EgrZFP6, which had lower transcripts level in leaves than in roots and stems. When treatedwith different abiotic stresses, expression of EgrZFP1-7were induced by low temperature,2C or4C was the optimal temperature for increasing the transcripts level of EgrZFPs. Time course ofEgrZFPs at4C for2h,4h,8h,24h and48h revealed EgrZFP1-6reached the highest level after48htreatment while EgrZFP7showed the transient expression pattern. Salinity (NaCl,200mM) alsoincreased expression of EgrZFP1-6, but inhibited EgrZFP7. All seven genes exhibited no response toABA (100μM), implicating they were independent to the ABA stress responding pathway. The resultsindicated that EgrZFP1-7are involved in cold and salt tolerance in Eucalyptus grandis, via aABA-independent way.Some transcription factors binding to the promoter of EgrZFP1were isolated by yeast one-hybridexperiments. The main TFs are as follows: transcription factor NAC with NAM domain,β-galactosidase, ATPase, aquaporin, Photosynthesis protein and two protein without annotation. Theexpression analysis of gene P3-1,4,5shows that gene P3-1that was translated to NAC wasupregulated by cold and salt stress, while its expression level was no obvious change by ABA anddrought treatment. Gene P3-4,5were upregulated by drought stress, while its expression level was noobvious change by ABA, cold and salt treatment. The results indicated that the three genes are involvedin abiotic stress tolerance via a ABA-independent way.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Research of the High-Rise Building’s Form and Expression in the New Century,TU971
  3. Research on Design of Teaching Buildings for Arts Departments of Higher Learning Institutions,TU244.3
  4. Research on Temporal Information Recognition and Normalization,TP391.1
  5. Expression of D-AtCGS in E. Coli and Preparation of Polyclonal Antibody Against D-AtCGS,Q943.2
  6. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  7. The Study on Arabidopsis Thaliana Heat Shock Factor HSFA 1d Response to Formaldehyde Stress,Q945.78
  8. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  9. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  10. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  11. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  12. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4
  13. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  14. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  15. Characteristics of Over-Expression for Nitrate Transporter Genes OsNRT1.2 and OsNRT1.5 in Rice,S511
  16. The Reaching of Humanism Foundation in the Junior Physics Teaching,G633.7
  17. Cloning Andanalysis of NAC Transcription Factor Gene in Tobacco(Nicotiana Tabacum L.),Q943.2
  18. Isolation and Identification of Infectious Bronchitis Virus and Sequence Analysis of Its S1 Gene and N Gene,S852.65
  19. Isolationand Identification of Porcine Parvovirus and Parts of Its Biological Characteristics,S852.65
  20. Studies on Construction of Specific Gland Expressional Vector of HLF and Modified Fibroblast Cell Line,Q78
  21. Pathogenic Mechanism of Invasion Associated Genes in Avian Pathogenic E. Coli Duck Isolate DE205B,S852.61

CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Eucalyptus
© 2012 www.DissertationTopic.Net  Mobile