Dissertation > Excellent graduate degree dissertation topics show

Isolation and Identification of Pediococcus and Study of Silage Inoculant

Author: SuiHongYuan
Tutor: ZhouYuXia
School: Inner Mongolia Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Lactic acid bacteria Isolated Identified Screen Silage inoculant
CLC: S816.53
Type: Master's thesis
Year: 2013
Downloads: 3
Quote: 0
Read: Download Dissertation

Abstract


Lactic acid bacteria were isolated and identified from the silage of Inner Mongolia Hohhot small dam pasture and milk cooperative farm by conventional bacteriological methods.10strains of Pediococcus were successfully screened from silage samples of different sources, including2strains of P. pentosaceus and8strains of P. acidilactici.By the temperature growth test, the test of producing acid, acid resistance, bile salt tolerance, three stains were respectively chose from8strains of Lactobacillus plantarum,7strains of Enterococci, which was preserved in microbal lab, and10strains of Pediococcus, which were L. plantarum48-2, E. faecalis44-1and P. acidilactici P-1.3stains of the lactic bacteria can be growed at25-37℃, pH4.0-5.0,4%-6%NaCl, and used the carbohydrate of the silage, and had good bacteriostatic effect to E. coli.3stains of the lactic bacteria were studied as silage inoculants. Results of the same raw materials and different combination of lactic acid bacteria showed that:Either the experimental group or the control group, both the viable count of the lactic acid bacteria and the silage temperature reached the highest value on the third day in silage. During ensiling, adding three strains of lactic acid bacteria, the viable count of the lactic acid bacteria was superior to adding two kinds of lactic acid bacteria and control group (P<0.05), pH values was lower than that of adding two kinds of lactic acid bacteria and control group (P<0.05). According to the silage evaluation, it showed that3kinds of lactic acid bacteria as silage inoculants had improving effect on silage.By the study of compound silage inoculant consisting of3lactic acid bacteria to the silage effect of the silage materials from different sources, the results showed that:The viable count of the experimental group was significantly superior to the control group (P<0.05). pH values was significantly lower than that of the control group(P<0.05). Both the experimental group or the control group, the viable count of lactic acid bacteria and pH values were not significantly different. The results showed that compound silage inoculant consisting of3lactic acid bacteria had no different on the silage effect of different silage materials, but it had improving effect on silage quality of different silage materials.

Related Dissertations

  1. Design of Electronicalcontrol System for Electronic Jacquard Knitting Machine,TS183
  2. Fabrication of Membrane Electrode Assembly for PEMFC,TM911.4
  3. Isolated systolic hypertension in patients with cardiac ultrasound characteristics of traditional Chinese medicine dialectical correlation exploration,R544.1
  4. Micro- grid with distributed power control strategy research,TM61
  5. Using an F2 Screen to Estimate the Frequency of Resistance Alleles to Cry1Ab in Wenzhou Population of Chilo Suppressalis and Cloning of an Aminopeptidase N Gene CsAPN3,S435.112.1
  6. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  7. Using an F2 Screen to Estimate the Frequency of Resistance Alleles to Cry1Ab in Wenzhou Population of Chilo Suppressalis and Cloning of an Aminopeptidase N Gene CsAPN3,S435.112.1
  8. Screening, Identification of Antagonistic Organisms Against Phytophthora Capsici and Effect of Their Bio-Organic Fertilizer on Pepper,S436.418
  9. Studies on Screening in Progeny Population of Distant Hybridization between Brassica Napus L. and Descurainia Sophia,S565.4
  10. Soy Germ Isoflavone Conversion by Lactic Acid Bacteria and Development on Its Chewing Tablet,TS214.2
  11. Studies on Breeding of Tetraploid in Pakchoi (Brassica Rapa L. Ssp. Chinensis L.) Based on Microspore Culture,S634.3
  12. The Processing Technology Studying of Fermented,TS251.65
  13. Isolation and Identification of Dominant Lactic Acid Bacteria from Cured Fish and Study on DVS Production Technology,TS254.4
  14. The Effect and Significance of Combination Chemotherapy in the Expression of CD90 and CD44 in Human Hepatoma Cell Line HepG2,R735.7
  15. Non- rigid body system kinematics and dynamics of the collision point,O313.3
  16. PLC Control System Design of the Wet Spraying Machine,TU69
  17. Two-Point Resistive Touch Screen Design,TN873
  18. Study on Capacitive Touch-screen,TN873
  19. Research on Now-casting Technique for Severe Convective Weather in Ningbo,P456
  20. Detection of Brucella and Listeria Monocytogenes(LM) in Raw Milk by Duplex PCR ,antibody Screening on Four Major Animal Diseases in Hanzhong Area,R155.5
  21. Screen Surface in the New Media Technologies to Enhance the Quality of Information Communicated,J524

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed > Roughage > Silage
© 2012 www.DissertationTopic.Net  Mobile