Dissertation > Excellent graduate degree dissertation topics show

Study on the Diversity and Distribution of the Pico-Chlorophyta and the Pico-Haptophyta in the East China Sea

Author: LiaoYu
Tutor: HuangBangQin
School: Xiamen University
Course: Environmental Science
Keywords: Prasinophyceae Haptophyta Genetic Diversity Temporal and SpatialVariation 18S rRNA FISH-TSA the East China Sea
CLC: S917.3
Type: Master's thesis
Year: 2014
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


The Chlorophyta and the Haptophyta are the important primary producers of marine ecosystem, play an important role in the material circulation and energy flow. But the knowledge of their diversity and distribution is very limited. In this study, we used molecular approaches,18S rRNA clone library and fluorescent in situ hybridization associated with tyramide signal amplification (FISH-TSA) to investigate the diversity of Chlorophyta and Haptophyta and the distributions of major components of the photosynthetic picoeukaryotes in the East China Sea. The main results are as below:(1) We chose8samples from6stations in the East China Sea and the source area of the Kuroshio in the West Pacific to construct the18S rRNA clone libraries for Chlorophyta and Prymnesiophyceae.The Chlorophyta had a high diversity in all the samples and we got336Chlorophyta sequences which could be classified into35operational taxonomic unit (OTU). The sequences fall into5groups:Pyramimonadales, Mamiellophyceae, Prasino-Clade VII, Prasino-Clade IX and Trebouxiophyceae. The phylogenetic analysis showed the sequences all fall into the known evolutionary branch. The Micromonas Clade A and Ostreococcus Clade B were the dominate groups of the Chlorophyta in the East China Sea, the Micromonas Clade B._.4contain sequences only from stations in the continental shelf. An Ostreococcus OTU and a reference sequence from the Red Sea formed a new evolutionary branch, could be a new Ostreococcus clade.We got554Prymnesiophyceae sequences which divided into58OTU. The sequences fall into6groups:Phaeocystales, Prymnesiales, Hapto-Clade D, Hapto-Clade E, Zygodiscales and Syracosphaerales. Phylogenetic analysis showed the sequences fall into the known evolutionary branches and a new evolutionary branch. The sequences of this branch all came from the West Pacific libraries suggest this branch could be a new Haptophyta group that mainly distributed in the oligotrophic waters. The Hapto-Clade D was thought to be widely distributed in the oligotrophic waters, but our results showed the Hapto-Clade D could also existed in the mesotrophic or even the eutrophic waters.(2) We investigated the temporal and spatial variations of the major components of photosynthetic picoeukaryotes (PPEs) in spring and fall in the East China Sea.Results suggested the pico-Chlorophyta and the pico-Haptophyta were the major components of the PPEs which account for44.8-100%of the total cell abundance in the spring and fall cruises. The max abundance of PPEs in the spring cruise was36364cells mL-1, significantly higher than that of the fall cruise which was18715cells mL-1. In the fall cruise, the PPEs showed a patchy distribution along the estuary-continental shelf-open sea, while it was mainly distributed at the Changjiang estuary area and the pico-Haptophyta mainly distributed at the surface layer of continental shelf in the spring cruise.

Related Dissertations

  1. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  2. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  3. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  4. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  5. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  6. Genetic Diversity and Ultrastructural Studies of Southern Corn Rust in China,S435.131.4
  7. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  8. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  9. Molecular Characterisation Based on ITS and Genetic Diversity of the Heterodera Avenae Group on the Huang-huai Floodplain of China,S435.121
  10. Characterization of Population Structure and Linkage Disequilibrium of Chinese Soybean Landerace Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  11. Genetic Diversity, Genetic Variance Association Mapping of Fresh Seed Quality Traits QTLs of G.Max in China,S565.1
  12. Studies on Genetic Diversity and Phylogenetic Relationship of Dendranthema and Its Related Genera,S682.11
  13. Polymorphisms of Y-STRs in Uygur and Kazak Ethnic in Xinjiang,R394
  14. The Research on Population Characters and Evaluated the Genetic Diversity of Chinese Wild Pyrus Ussuriensis Maxim.,S661.2
  15. The Genetic Diversity of 12 STR Loci in Four Dog Breeds and the Application in Individual Discrimination,S829.2
  16. Study on Molecular Systematics of Bangia Atropurpurea,Q941
  17. Study on Diversity of CHS Gene from Buckwheat Germplasm Resources,S517
  18. Genetic Diversity in Different Populations of Tilapia (Oreochromis) by Microsatellite DNA Analysis,S917.4
  19. Genetic Diversity of Chimonanthus Praecox (L.) Link Based on ISSR Markers,S685.99
  20. The Research of Molecular Genetics about Carassius Auratus in Qihe River by ISSR and Cytb,S917.4
  21. The Study of Genetic Diversity and Molecular Phylogenetic of Acheilognathinae,S917.4

CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Botany
© 2012 www.DissertationTopic.Net  Mobile