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Study on Large-Scale Artificial Breeding Technology of Cyclina Sinesis

Author: ZhangMeiRu
Tutor: ZhuJian
School: Nanjing Agricultural College
Course: Agricultural Extension
Keywords: Porphyra yezoensis Germ Amplification Conservation Contamination control
CLC: S968.431
Type: Master's thesis
Year: 2010
Downloads: 11
Quote: 0
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Abstract


Porphyra yezoensis is a species existing at the western part of North Pacific. In China, it grows at northern part of the East China Sea to the north of Zhoushan islands and the coast of the Yellow Sea and the Bohai Sea. Porphyra yezoensis is a main species for cultivation to the north of the Yangtze River. There is a typically heterogamous life history which includes a macroscopic foliose phase and a microscopic conchocelis phase in Porphyra. And free-living conchocelis is the optimum form for germ conservation and fine germ application. During the fine germ management in Porphyra yezoensis, the existence of contamination has seriously affected fine germ research and application, even led to the lost of fine germ. So the contamination control technology in germ amplification and conservation of Porphyra yezoensis has been researched.To prevent contamination in germ amplification of Porphyra yezoensis, different illumination and temperature impact on preventing conchosporangial branch from forming in germ fast amplification, illumination impact on microalgae contamination, GeO2and other chemicals impact on diatoms contamination, penicillin impact on bacteria contamination have been researched. Several results have been gained. Conchosporangial branch could be prevented at the temperature of15-20℃, illumination of<3000Lux, photoperiod of12L:12D to18L:6D. It could effectively restrain microalgae multiplying under the illumination of less than1500Lux. The treatment of NH4HCO32000mg/1with96h is useful in greenalgae contamination control, while CuCl2300～500mg/1with96h could reduce the degree of greenalgae contamination. It is useful in preventing diatoms contamination to add3mg/1GeO2into the culture solution in germ amplification and the treatment of9mg/1GeO2with96h could effectively get rid of diatoms. Bacteria could be effectively eliminated by the adjunction of penicillin with300,000U/1000ml in culture solution for72h. To prevent contamination in germ conservation of Porphyra yezoensis, contamination control in germ preparation, impure germ purification, the best occasion in which conchocelis being taken to conservation process, GeO2impact on diatoms contamination and illumination impact on micro green algae contamination have been researched. Several results have been gained. In the process of germ preparation, the tissues should be frozen under-20℃for more than15days or exposured under ultraviolet ray (15W) for10min, and then be washed by1%citric acid for2min to prevent contamination. The optimum temperature when conchocelis was taken to conservation process is about5℃. Isolated rooms and special storages should be set for germ conservation with special administers. The germ should be purified by culture on shells or on agar medium in the situation of serious contamination. It is useful in preventing diatoms contamination to add2-3mg/1GeO2into the culture solution in germ conservation and the treatment of dark could restrain micro green algae multiplying.Through this research, the contamination in germ management of Porphyra has been effectively controlled. The degree of contamination in germ conservation has been reduced by about80%and the efficiency of germ amplification has been raised by more than20%. The provincial standard "operating instruction of germ conservation in Porphyra yezoensis" has been revised according to the results of this research which have improved germ management technology and operating procedure, provided germ resource effective protection and fine germ amplification and application with scientific practical technology.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquaculture technology > A variety of seafood, animal and plant breeding > Algae farming > Red algae > Laver
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