Dissertation > Excellent graduate degree dissertation topics show

Acupoint catgut embedding stem mechanism in expression of nestin, GAP-43 Road, the number of retinal ganglion cells and optic nerve injury in rats with preview

Author: QianJingLi
Tutor: FengLingMei
School: Guiyang College of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: TON acupoint catgut embedding therapy Nerve protection Thevisual pathway Retinal ganglion cells nestin GAP-43
CLC: R245
Type: Master's thesis
Year: 2013
Downloads: 11
Quote: 0
Read: Download Dissertation

Abstract


Objective:This experiment use the traditional Chinese medicine therapy-acupoint catgut embedding therapy to treat optic nerve injury model in rats and observe the changes of rat retinal ganglion cells and the relationship between the changes and damage time. In this way can provide laboratory basis for mechanism of acupuncture treatment for traumatic optic nerve injury.Methods:208rats are randomly divided into6groups:blank group, control group, model group, acupoint catgut embedding therapy group, neurotrophic factor group and acupoint catgut embedding therapy with neurotrophic factor group. Among the last five groups, they are divided into5subgroups:4days group,7days group,14days group,21days group and28days group. Making the traumatic optic neuropathy rats with transactional injury. After catgut embedding on the Ganshu (BL18), observing the morphology of retina and numbers of the retinal ganglion cells, and detecting the expressing situations of nestin and GAP-43in LGN with immune histochemical method after therapy.Results:Compared with the model group, the retinal ganglion cells are rising in acupoint catgut embedding therapy group, neurotrophic factor group and acupoint catgut embedding therapy with neurotrophic factor group (P<0.05or P<0.01). Compared with the model group, the expression of nestin and GAP-43in LGN is higher in acupoint catgut embedding therapy group, neurotrophic factor group and acupoint catgut embedding therapy with neurotrophic factor group (P<0.05or P<0.01).In the early damage, the number of RGC in acupoint catgut embedding therapy group is more than neurotrophic factor group (P<0.01). But14days later, the results are just the opposite.Conclusion:1. acupoint catgut embedding therapy is an effective treatment in treating the optic nerve injury rats. Using the acupoint catgut embedding therapy, The early effect is effective. But after14days neurotrophic factor is better.2. We speculated that the mechanism of treating TON by acupoint catgut embedding therapy is reducing the mortality of RGC, raising the expression of nestin and GAP43. Activate the neural stem cells in the central nervous system inorder to promote the growth of axons.3. When treating TON, combine traditional Chinese and western medicine treatment is not as good as point embedding therapy or neurotrophic treatment.

Related Dissertations

  1. Effect of Microwave on GAP-43 Expression in the Spinal Cord after Sciatic Nerve Lesions in Rats,R651.3
  2. Effects of Erythropoietin on the Expression of 5-bromo-2-deoxyuridine and Nestin in Neonatal Rat Brain after Hypoxic-ischemic Brain Damage,R722.1
  3. Study of the Effects and the Mechanism of High Intensity Pulsed Electromagnetic Stimulation on Proliferation of Neural Stem Cells of Neonatal Rat in Vitro,R329
  4. Effects of Magnetic Stimulation on the Expression of GAP-43 after Spinal Cord Injury,R651.2
  5. α crystal protein and retinal ganglion cell membrane associated proteins of exploration and study,R774.6
  6. Correlation Analysis of Firing Activities of Retinal Ganglion Cells,R318.0
  7. The Effects of Progesterone on Nogo-A、GFAP and GAP-43 Protein Expression in Rat Brain after Traumatic Brain Injury,R965
  8. The Effects of Nogo Receptor Inhibitor on Nerve Regeneration in Rat Brain after Diffusive Axonal Injury,R965
  9. The Experimental Study on Estrogen Therapy of Spinal Cord Injury,R651.2
  10. Expression and Significance of Mst3b after Spinal Cord Injury in Adult Rats,R651.2
  11. Long-term Effects of Intravitreal Injection of Erythropoietin Sustained-release Microspheres on Retinal Ganglion Cells after Optic Nerve Crush in Adult Rats,R774.1
  12. The Effect of Yuxianling Granula on the Learning and Memory, Hippocampus MFS of KA Induced Temporal Lobe Epilepsy in Rat,R742.1
  13. The Influence of Expression of Arc、GAP-43 and P38 on Axonal Sprouting and Synaptic Plasticity in Hippocampus of Insular Epilepsy Rats,R742.1
  14. Insulin Prevents Ischemia/reperfusion-induced Injury in Cortical Neuron of Rat,R743.3
  15. The Stem Cell Antigen Phenotype of Glial Cells,R329
  16. Benxi Iron and Steel Group Energy Audit,F206
  17. Study on Hot Metal Temperature Drop on BF/BOF Region in Iron & Steel Factory,TF703.7
  18. Analysis on Energy Intensity of Shougang Output-cut Project in 2008,F426.31
  19. Benxi Iron and Steel Group Energy Saving Plan,F206
  20. Expression of Stem Cell Markers CD133、Nestin and CD44 in Malignant Melanoma and Its Significance,R739.5
  21. Studies on the Active Polysaccharides Constituents from Sea Cucumber Apostichopus Japonicus,S917.4

CLC: > Medicine, health > Chinese Medicine > Chinese medicine clinic > Acupuncture, acupuncture therapy
© 2012 www.DissertationTopic.Net  Mobile