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Study on plant Elephantopus and Gerbera Guangxi Jingxi Zhuang medicine, Dragon Boat

Author: WangJianJun
Tutor: LongChunLin
School: Central University for Nationalities
Course: Ethnoecology
Keywords: Ethnobotany Elephantopus scaber Gerbera piloselloides Antioxdation Cytotoxicity Target fishing
CLC: R29
Type: Master's thesis
Year: 2013
Downloads: 18
Quote: 0
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Abstract


With the deficiency of combinatorial chemistry in new drug research and the rapid development of isolation and identification technology in natural products, natural product research appears to be claiming more attention in new drug research. Meanwhile, more and more attentions have been put on traditional medicinal plants and medicinal knowledge, as the important resource of natural product research. The rich resources of traditional knowledge on medicinal plant accumulated from thousands years ago in china, supported great potential for the development of natural product research in our country.In this paper, the ethnobotany method including key information interview, semi-structure interview and participatory rural appraisal was used to invest the various traditional knowledge of medicinal plants on dragon-boat festival, Jingxi County, which consist of the traditional usage, preparation, using parts and so on. After knowing the widely use and good treatment effects of E. scarber and G. piloselloides, this two plants were selected to conduct the phytochemistry research in lab. Furthermore, phytochemistry technology (Silica gel chromatography, Sephadex chromatography, HSCCC, HPLC and others methods) was used to isolate the compounds from the extract of this two meicinal plants. Meanwhile, spectra identification technology (NMR, MS and IR) was used to determine the structure of compounds. Finally, bio-activities experiments including ABTS radical scavenging assay and cytotoxicity assay, and computer virtual activities screening were applied to detect the bioactivities of some compounds isolated. The results was expected to test the rationality of traditional knowledge of E. scarber and G. piloselloides, provide scientific basis for the inheritance and protection of traditional knowledge and put the foundations for the further research of this two plants.Using various column chromatography technologies, such as gel, sephadex and MCI chromatography,8compounds belonged to δ-truxinate, sesquiterpene lactone and flavonoid types, were isolated from E. scarber, and the structures were identified by NMR, MS and IR spectrum technology. Compound8was determined as a new δ-truxinate natural product. The antioxidation activity of some compounds isolated were detected by ABTS radical scavenging assay using VE as positive group, which shown that this new compound has strong antioxidation potential (IC50=0.57mg/mL). Furthermore, the MTT method with norcantharidin (NCTD,10.0mg/mL) as positive group was used to detect the cytotoxicity of compound8against HepG2. The MTT results showed that this compound has dose-dependent inhibition activity on HepG2cell (IC50=24.0mg/mL). Finally, this paper used the target fishing function of Discovery studio software connected with PDB database to predict the bioactivities of this compound, which indicated that it has anti-inflammatory, anti-biotics and antitumor activities.In this paper, using ABTS antioxidation activity-guided method and combining macroporous resin chromatography, sephadex chromatography and HPLC, ten compounds were isolated from G. piloselloides, which belongs to flavonoids, quinic acids derivatives and coumarine types compounds. The structures of these compounds were determined by NMR and IR spectum technology, which shown that compound5belonged to parasorbosid is new natural product isolated from plant for first time. The antioxidation activities of these ten compounds were detected using ABTS radical scavenging assay, and the IC50value was obtained, which can provide foundation for further study. Meanwhile, the taxonomic significances of above experimental results on Gerbera and Piloselloides genus was discussed, which supported preliminary evidence for the chemotaxonomy on this two genus.

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