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Effects of Procyanidin on Cardiocyte Apoptosis after Myocardial Ischemia Reperfusion in Rats

Author: LiuDan
Tutor: LiuYi
School: Liaoning Medical
Course: Pharmacology
Keywords: ischemia reperfusion procyanidin signal transduction cardiocyte apoptosis
CLC: R285.5
Type: Master's thesis
Year: 2013
Downloads: 5
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Abstract


ObjectiveTo evaluate the effects of procyanidin on cardiocyte apoptosis ofmyocardial ischemia-reperfusion injury in rats and its probable mechanism.MethodsFourty male SD rats were randomly assigned to four groups: shame group，ischemia and reperfusion group，low-dose group（procyanidin50mg·kg－1）and high-dose group（procyanidin100mg·kg－1）．The rats were pretreated byintragastric administration for2weeks. All rats，except for those in shamegroup，received occlusion of left anterior descending artery (LAD) for30minutes followed by120minutes of reperfusion．Serum CK－MB were detectedafter experiment．The ROS fluorescent probe dihydroethidium was used for themeasurement of ROS．The expression of p53, caspase-9was determined bywestern blotting. The myocardial apoptotic index was measured by the methodof TUNEL．ResultsCK-MB: Ck-mb activity increased significantly in the I/R group Comparedwith the shame group. Compared with the I/R group, PC50and PC100reducedck-mb activity, while ck-mb activity in PC100group was lower than the PC50group.ROS: ROS activity increased significantly in the I/R group Compared with the shame group. Compared with the I/R group, PC50and PC100reduced ROSactivity, and ROS activity in PC100group was lower than the PC50group.P53and caspase-9: The exppression of P53and caspase-9was increasedin the I/R group compared to the shame group, and PC50and PC100decreased the expression of P53and caspase-9compared with the I/R group，and，PC100decreased the expression of P53and caspase-9compared with thePC50group.Apoptotic Index: AI increased significantly in the I/R group compared withthe shame group. Compared with the I/R group, PC50and PC100reduced AI,while AI in PC100group was lower than the PC50group.ConclusionProcyanidin can reduce reduced apoptosis index afterischemia-reperfusion． This beneficial effect is partially dependant onROS-p53-caspase-9signal transduction pathway．

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