Dissertation > Excellent graduate degree dissertation topics show

Material Basis of Ginseng and Radix Aconit:Praeparata Compatibility to Attenuate Toxicity

Author: ZhouSiSi
Tutor: MaZengChun
School: Anhui Medical University,
Course: Pharmacology
Keywords: Shenfu formulation compatibility attenuation toxicity material basis
CLC: R289.1
Type: Master's thesis
Year: 2013
Downloads: 57
Quote: 0
Read: Download Dissertation

Abstract


Safety incidents of Traditional Chinese medicine (TCM) take place frequently, which become an important obstruction of the modernization development of the TCM, and put forward a new challenge to preclinical evaluation of the TCM in the process of new drugs foundation and clinical drugs development. Shenfu formulation which composed of ginseng and radix aconiti praeparata, has exact curative effect to treat heart failure, while it has adverse reaction such as shock, allergic reactions. In the present study, the influence of indicative components resulted from decocting time and compatibility on efficacy and toxicity of Shenfu decoction were investigated, in order to provide scientific basis for optimization design components of Shenfu decoction, to clarify the compatibility connotation, which is helpful to establish the research model of material basis analysis of compatibility attenuation.The uniform design and fixed dose of radix aconiti praeparata compatibility with ginseng were used to observe the influence of different proportion compatibility on acute toxicity, to find appropriate dose of ginseng and radix aconiti praeparata. Then alkaloid and ginsenoside were investigated, finally, chemical constituents absorbed in rat blood were studied, in order to clarify material basis to decrease toxicity in Shenfu formulation in vitro and in vivo.The study of uniform design showed that mice mortality increased with the dose of radix aconiti praeparata while decreased with the dose of ginseng. The research of fixed LD50dose of radix aconiti praeparata and different proportion of ginseng showed mice mortality decreased with the dose of ginseng in a certain range, especially the dose of ginseng more or equal to the radix aconiti praeparata. The delimitation point of attenuated toxicity was the dose of ginseng equal to the radix aconiti praeparata, which provided reference for clinical application. The content of diester-diterpenoid alkaloid such as aconitine, mesaconitine hypaconitine was higher during2-10min, while the content of monoester-diterpenoid alkaloids such as benzoylaconine, benzoylmesaconine, benzoylhypaconine increased during the first60minutes. A suitable processing time should be chosen according to clinic indications in order to attenuate toxicity and increase efficiency. Within30minutes is the best time for diester-diterpenoid alkaloids and60minutes for monoester-diterpenoid alkaloids as pharmacodynamic material of radix aconiti praeparata. Results showed that there were significant difference between co-decoction and mixed decoction of ginseng and radix aconiti praeparata in positive ion mode, the content of hypaconitine, deoxyaconitine decreased, while the content of benzoylmesaconine, benzoylhypaconine, dehydrated benzoylmesaconine increased in samples of ginseng and radix aconiti praeparata co-decoction. The content of diester-diterpenoid alkaloids decreased and the monoester-diterpenoid alkaloids increased, which may be the basis of chemical composition in combination of ginseng and radix aconiti praeparata. At the same time with the incompatibility of radix aconiti and pinellia praeparata as evidence to synergistic shenfu formulation. The results of positive ion mode showed that there were significant difference between two kinds of decoction samples, the content of hypaconitine, mesaconitine, aconitine, deoxyaconitine,10-OH-mesaconitine,10-OH-aconitine increased, while the content of mesaconine, dehydrated benzoylmesaconine, dehydrated benzoylhypaconine, benzoylaconine, benzoylhypaconine,10-OH-benzoylmesaconine decreased in samples of radix aconiti and pinellia praeparata co-decoction. The content of diester-diterpenoid alkaloids increased, while the content of monoester-diterpenoid alkaloids decreased, which may be validate reduction toxicity effect of Shenfu formulation in the opposite side. Results showed that there were significant difference between co-decoction and mixed decoction of ginseng and radix aconiti praeparata in negetive ion mode, the content of (20R)-ginsenoside Rhl, ginsenoside Ⅳa increased, while the content of barley-sugar decreased in samples of ginseng and radix aconiti praeparata co-decoction. The content of ginsenoside increased, which may be the basis of chemical composition in combination of ginseng and radix aconiti praeparata. At the same time with the compatibility of ginseng and radix ophiopogonis as evidence to synergistic shenfu formulation. Results showed that significant difference between two kinds of decoction samples, the content of ginsenosideRgl, ginsenosideRe, ginsenosideRb1, malonylginsenosideRb1decreased, while ginsenosideRb3, malonylginsenosideRb2, malonylginsenosideRc, ginsenosideRf increased in samples of ginseng and radix ophiopogonis co-decoction.The content of protopanaxatriol type ginsenoside decreased, while protopanaxadiol type ginsenoside increased, which may be validate increasing effect of Shenfu formulation from another side. Finally, study of Shenfu injection on serum chemistry indicated that ginsenoside Rd, ginsenoside Rg1, ginsenoside Ro maintained in blood for a short time, while ginsenoside Rbl, ginsenoside Rb2, ginsenoside Rc for a long time.The present study discussed the effects of preparation conditions and preparation methods on component indexes in the quality and quantity level, the toxic constituents fingerprint and dose-toxicity relationship between radix aconiti praeparata and ginseng were discovered. Diester diterpenoid alkaloids were found to be the main toxicity components of Shenfu formulation. We will investigate the role of diester diterpenoid alkaloids to reduce toxicity and increase curative effect, reveal the mechanism of attenuation toxicity of compatibility, clarify the scientific connotation of compatibility.

Related Dissertations

  1. Synthesis and Biocompatibility of different types of hydrophilic surface - modified polyurethane,R318.08
  2. The application of nanotechnology in the field of anti - coagulation of biological materials,R318.08
  3. Synthesis and properties of new functionalized graphene oxide drug carriers,TQ460.4
  4. Synthesis and Properties of the new anti-clotting material in modified ammonium phosphate zwitterion,R318.08
  5. Preliminary Study on the Fertility Performance of Cucumis Hytivus Chen and Kirkbride and Breeding of Gynoecious Cultivated Lines,S642.2
  6. Cross Compatibility and Rapid Propagation Technology Research of Hemerocallis Middendorfii,S682.19
  7. Study on Cross-compatibility and Embryology of Interspecific Hybridization in Iris,S682.19
  8. Study HAC Solution for Mobile Phone Antenna,TN828.6
  9. Research on Staff Scheduling in the Call Center of Gansu Mobile,F626
  10. A Study on the Biodegradable β-TCP/PLLA Nanocomposites by Self-modification,TB332
  11. The Research on PLA/PE/Starch Blends,O631.3
  12. Power supply filtering components of broadband,TN713
  13. Preparation and Properties Study of PC/Bulk ABS Alloys,TQ325.2
  14. Research on the EMC and Cogging Torque of a Small Moment Gyroscope,TN03
  15. Comparative Study on Therapeutic Effect and Its Mechanism of Coptidis Rhizome, Rehmannia Dride Rhizome and Their Compatibility on Type 2 Diabetic Mellitus Rats,R285.5
  16. A high-power pulse modulator structure design and exploration,TN761
  17. Vacuum circuit breaker module power system electromagnetic compatibility and reliability design,TM561.2
  18. EMC Design and Test Server,TN03
  19. Study on the Eco-compatibility between Coastal Wetland Nature Reserve and Port Construction,X37
  20. Formation of Singularities for Vibrating String Equation of Mixed Initial-boundary Value Problems with Inhomogeneous Neumann Conditions,O175.8
  21. Structural design for UV-curable acrylic resin coatings and inks,TQ630.4

CLC: > Medicine, health > Chinese Medicine > Traditional Medical Formulae > Prescriptions of the
© 2012 www.DissertationTopic.Net  Mobile