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Molecular Display Based on the Nanoparticles Used in the High Sensitive Detection

Author: ZhangLiSha
Tutor: ShiYuanYuan
School: Henan University
Course: Biochemistry and Molecular Biology
Keywords: nanoparticles molecular display high sensitivity detection
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Type: Master's thesis
Year: 2013
Downloads: 32
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Abstract


Medical Laboratory technology plays an important role in the prevention and diagnosis of disease.While, the molecular display based on nanoparticles provides a powerful method for the MedicalLaboratory Technology; and thus, the low abundance medical indicators can be detected.Objective: hepatitis B virus (HBV) virus-like nanoparticles and ferritin clathrate nanoparticleshave been engaged in the displaying of the detection-associated molecules. Display the detection-associatedmolecules onto the nanoparticles to build the functional compound nanoparticles for the high sensitivedetection. And bring these functional compound nanoparticles into the detection of humanimmunodeficiency virus type1(HIV-1) P24antigen to realize its high sensitivity detection.Methods: In this study, by means of molecular biology, combine the HBV virus-likenanoparticles with the Staphylococcus aureus Protein G (SPG) which can bind to antibody wildly to buildan antibody-broad-binding HBV virus-like nanoparticle. Put this composite nanoparticle into the detectionof HIV-1p24antigen, and detection sensitivity should be tested; display the biotin-binding peptide (BAP)displayed on the ferritin clathrate nanoparticles and through biotinylating to construct a streptvidin-bindingferritin clathrate nanoparticle. And applicate it into the detection of the HIV-1p24antigen, test its relativedetection sensitivity.Results: Successfully builded the antibody-broad-binding HBV virus-like nanoparticle. And withthe using of the this nanopaticle in the detection of HIV-1P24, the detection lower limit can reach1ng/mL,compared to the detection lower limit of commercial HIV-1P24ELISA kit10ng/mL, the detectionsensitivity have been improved significantly; Successfully builded the streptvidin-binding ferritin clathratenanoparticle. And with the using of the this nanopaticle in the detection of HIV-1P24, the detection lowerlimit can reach1ng/mL, compared to the detection lower limit of commercial HIV-1P24ELISA kit10ng/mL, the detection sensitivity have been improved significantly.Conclusions: the technical platform based on the HBV virus-like nanoparticles and ferritinclathrate nanoparticles has been established successfully. Further more, the SPG and biotin molecules havebeen displayed onto the nanoparticles separately; putting these compound nanoparticles into the detection of HIV-1P24have shown a high sensitivity, and also there is a significantly improvement of detectionlower limit compared to the commercial ELISA test.

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