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The Preparation of Stimu-response Doxorubicin Magnetic Nanoparticles and in Vitro Experiment

Author: GuJunTong
Tutor: XuWeiPing
School: Anhui University of Chinese Medicine
Course: Pharmacy
Keywords: disulfide glutathione response magnetic nanoparticles in vitro
CLC: R914
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
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Abstract


Objective:With doxorubicin（DOX） as a model drug, to prepare disulfide modifiedmagnetic nanoparticles as drug carrier for tumer treatment.Methods:Firstly, this research synthesized Fe3O4nanoparticles by using a high-temperature hydrolysis reaction, and then we use the method of poly-propylenealcohol method to synthesis SiO2@Fe3O4magnetic nanoparticles. Byamino-modification of positive charge at the SiO2surface, coupled model drugDOX.The drug loading rate and enecapsule efficiency were quantified byfluorescence. On the surface of SiO2@Fe3O4is coated by PMASHand PVP thatthrough hydrogen bonds, chloramine T forming disulfide bonds. NMR experiment ofS-S modified magnetic nanoparticles is to examin the feasibility of in vivo imaging;In different concentrations of glutathione, DOX/magnetic nanoparticles distinction invitro release; To determine medical materials biosafety, we do the hemolysis test ofthe nanoparticles; The effect of disulfide modified DOX/magnetic nanoparticlesstudy on C6, Hela cell in vitro experimental.Results: Study on preparation of superparamagnetic nanoparticles in the magneticfield, magnetic responsive with good, size manageable, about30nm. With TEOS asthe main silica source, we synthesized mesoporous silica on the surface of the Fe3O4by chemical reactions, approximately70nm average particle size. Nanoparticles witha large specific surface area and a positive charge, DOX/magnetic nanoparticles drugloading rate of30.18%, enecapsule efficiency is65.84%. Through magneticresonance imaging experiments suggest that disulfide modified DOX/magneticnanoparticles have a potential for in vivo imaging. Hemolytic experiments showedthat conform to safety requirements of medical materials. C6, Hela results indicatethat tumor cells in vitro, disulfide-modified DOX/magnetic nanoparticles consist of10mMGSH group and0mMGSH group that has significant survival differencesstatistically significant. After co-culture with10mMGSH, cell viability of this twokinds of cells are significantly lower than the0mMGSH group. Conclusion: Disulfide modified DOX/magnetic nanoparticles response in simulationrestores glutathione concentration inside the cell, control releasing of DOX. In vitroexperiments, comparison with0mMGSH groups, significantly reducing survival ofC6, Hela cells, provides a possible new formulations for the treatment of tumors,there are good prospects of clinical application.

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CLC: > Medicine, health > Pharmacy > Drug basic science > Medicinal Chemistry
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