Dissertation > Excellent graduate degree dissertation topics show

A Highly Efficient Method for GPCR Gene-targeted Vector Construction by Red Recombination System

Author: JiangLi
Tutor: LiuMingYao
School: East China Normal University
Course: Biomedical
Keywords: gene knockout gene targeting vector construction red recombinase GPCR family
CLC:
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
Read: Download Dissertation

Abstract


G protein coupled receptor family (GPCR) is consisted of a polypeptide chain containing7transmembrane domains. It accounts for3%-4%of the human genome. GPCR has widely physiological effects and it is one of the most popular drug targets. Now the biological function and ligand of most Adhesion-GPCRs is still unknown. so it is also named orphan receptor. A study on the biological function of Adhesion-GPCRs has great significance in basic theoretical research and drug potential target discovery research.Phylogenetic analysis of GPCRs shows that the Adhesion-GPCRs is an unique family. Adhesion-GPCRs are characterised by long N termini.The N termini domain are often consist of a range of protein domains found in cell adhesion proteins. Obviously the study for Adhesion-GPCRs is required a lot of work to do. So we constructed the gene-targeting vector of Gpr56, Bail, Gpr111and Gpr126by using our system. Using the vectors mentioned above, we can study those genes at the molecular genetic level based on the knockout mouse model.At present, based on the knockout mouse model to study the function of gene has become an important academic field. However, construction of targeting vector remains a time-consuming and technically challenging process with the traditional method of constructed targeting vector. Here we describe a highly efficient recombineering-based method by red recombination system. In this way, we can construct targeting vector highly efficient through two times of recombination. In addition, we improved the method of conditional targeting constructoion by red recombination system. The two LoxP sites can be more accurate inserted into the target position with this improved method.

Related Dissertations

  1. Biological Characterizations of the Mutant Strain with HOR2 Gene Deleted in Sacchromyces Cerevisiae,Q78
  2. Construction of Bm-Bacmid and Prokaryotic Expression of Porcine Circovirus Typ Ii’s Capsid,S852.65
  3. Study on formation process of NRAGE gene in atherosclerosis,R543.5
  4. Biological Characterizations of the Mutant Strain with SNF1 Gene Deleted in Sacchromyces Cerevisiae,Q78
  5. Functional Analysis of Protein Kinase CK2 in Magnaporthe Oryzae,S435.111.41
  6. Construction of Genetic Knockout System by Endophytic Bacteria 336X with Biological Control Ability,S476
  7. Characterization of FgCPKA Gene in Fusarium Graminearum,S435.121
  8. Ralationship between Complement 3 and Neuropthic Pain: An Experimental Study,R741
  9. Study on the Function of Ca2+/CaM Dependent Protein Kinase Genes of Magnaporthe Grisea,S435.111.41
  10. Functional Characterization of Fusarium Graminearum TEP1 Gene,S435.121
  11. Construction and Characteristic Analysis for the hrpX/hrpG Gene Knock-out Mutant of Xanthomonas Oryzae Pv. oryzae,S435.111.4
  12. Cloning and Functional Analysis of STK1K Gene Encoding a MAPK Kinase in Setosphaeria Turcica,S435.13
  13. Cloning and Functional Analysis of the Type 2A Phosphoprotein Phosphatase Gene in Setosphaeria Turcia,S435.131
  14. Knockout shikimic acid transporter protein -coding genes shiA impact on shikimate accumulation,Q943.2
  15. Construction and in Vitro Expression Study of Neuron-specific Vector with Cre Recombinase,R346
  16. Mechanisms of Cleft Palate Formation in Wnt5a Gene Knockout Mouse,R782.2
  17. Effects of Celastrol on Platelet Function and the MIF and MMP-9 Expressions in the Aorta of ApoE Gene Knockout Mice,R285.5
  18. Research on Deletion of Alcohol Dehydrogenase Ⅱ Gene from Pachysolen Tannophilus P-01 Capable of Co-fermenting Pentose and Hexose Released from Lignocellulose,TQ920.1
  19. Cloning and Characterization of a G-Protein γ Subunit Gene in the Chestnut Blight Fungus, Cryphonectria Parasitica,S436.64
  20. A Preliminary Study on the Knockout of Myostatinin Buffalo Somatic Cells,S823.83
  21. The Isolation and Culture of Fetal Fibroblast of Porcine and MSNT Gene Knockout,Q78

CLC: >
© 2012 www.DissertationTopic.Net  Mobile