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Wheat Fusarium is a worldwide wheat scab caused by pathogenic fungi, wheat scab (Fusarium head blight or scab) is the world's warm and humid and semi- humid areas of the widespread occurrence of a devastating disease in recent years, in Europe, Asia and North America, many have an outbreak . Is also a major wheat diseases in our country , so that a serious decline in wheat production . Fusarium fungi can infect wheat crops and corn to make it pathogenic , can also infect other plants , such as: rice, sorghum and other crops and ice grass, barnyard grass and green foxtail and other weeds . Wheat Fusarium mycotoxins produced deoxy snow DON , zearalenone corn , etc. are human, animal after eating can cause fever, vomiting , diarrhea and other symptoms after eating dams pregnancy can cause miscarriage . Currently , wheat Fusarium prevention mainly chemical fungicides . However, from an environmental point of view, through molecular biology research reveals wheat Fusarium pathogenesis and then guide cultivation of resistant varieties , for the prevention and treatment of wheat scab occurrence of great significance . In this study, the use of homologous recombination , using Split marker technology, to build a wheat Fusarium FgCPKA knockout cassette . Application of PEG -mediated transformation of protoplasts in a medium containing hygromycin to select transformants obtained . In order to obtain positive clones were isolated for single- cell transformants were again screened positive and negative PCR and Southern blotting confirmed positive transformants further observed phenotypic and functional studies . Δfgcpka the wild type strain of morphological a significant difference , knockout mutant Δfgcpka mycelium short , dense , growth rate is much smaller than the wild- type PH-1 isolates. Liquid medium in the CMC , Δfgcpka conidia produced significantly less than the number of PH-1 wild- type strain . Self- test showed that the knockout mutant Δfgcpka carrot medium can also produce small amounts of perithecia . Virulence testing , the corn must be wild-type strain PH-1 infection completely when mutant Δfgcpka almost no infestation corn . Show FgCPKA knockout mutants Afgcpka significantly reduced virulence , FgCPKA wheat Fusarium important virulence -related genes. This article aims to explore FgCPKA initial basic functions of genes , to elucidate the pathogenesis of wheat Fusarium provide clues .
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