Dissertation > Excellent graduate degree dissertation topics show

Impact of the Length of Variable Region of Class1Integron and Plasmid Elimination on Expression of Integrase in Bacterias

Author: FengYin
Tutor: WuYong
School: Central South University
Course: Clinical
Keywords: Gram-negative bacteria integron multi-drug resistance plasmid elimination
CLC: R446.5
Type: Master's thesis
Year: 2013
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


Objective:To study the relationship between class1integron and Multi-drug resistant bacteria in Gram-negative bacteria, to identify the mechanism of integrons by detect the different expression of intll gene in different variable regions of integrons and different ways of plasmid elimination.Methods:87strains of Pseudomonas Aeruginosa and101strains of Escherichia coli were isolated from the clinical departments. The drug sensitive tests were conducted by using the K-B method.25strains of.P. Aeruginosa with intll gene was grouped into2groups depending on the length of variable regions of their integrons, and the relative expression of intll gene of different variable regions were detected by Real-time fluorescent quantitative PCR, and the condition of drug-resistance for the bacteria in the2groups was compared. Ultraviolet ray, SDS and berberine hydrochloride were used to eliminate plasmids with class1integron, then the effect of elimination and the relative expression of intll gene was detected.Results:Both in Pseudomonas Aeruginosa and Escherichia coli, the rate of multi-drug resistance in bacteria with class1integron is much bigger than bacteria without class1integron. Among the clinical strains of P. Aeruginosa with intI gene, the bacteria with short variable region expressed more intI mRNA than the bacteria with long variable region, but was less resistant to antibiotics than the bacteria with long variable region. SDS had obvious effect on plasmid elimination and the expression of intI1was less; berberine hydrochloride had medium effect on plasmid elimination but the expression of intI1was more; ultraviolet ray presented weak capacity in plasmid elimination but it promoted the expression of intI1.Conclusions:Class1integron is closely related to multi-drug resistance in bacteria both in Pseudomonas Aeruginosa and Escherichia coli. The expression level of intI has related on the length of variable region in intgeron. Different ways of plasmid elimination have different regulation of the expression level of intI. There are17pictures,12tables and41refrences.

Related Dissertations

  1. The Correlation Between Drug Resistance and Some Drug Resistance Genes in Sixty-one Gallibacterium Anatis Isolates,S852.61
  2. Screen Genes Affected the Activity of Quorum Sensing Regulator VqmA and Functional Analysis of LysR and MFS Proteins in V.choleare,R378
  3. Detection of Class Ⅰ Integron in Gram-negative Bacteria,R446.5
  4. Antimicrobial Mechanisms of Several Natural Preservatives,TS201.3
  5. The Research of the Mechanism of Integron Mediated Drug-resistance in ESBLs-producing Strains,R378
  6. Analysis of Drug Resistance of Pathogens of Nosocomial Infections in Critically Ill Patients and Risk Factors,R446.5
  7. Aminoglycoside-resistant Pseudomonas Aeruginosa and the Molecular Mechanism of Drug Resistance,R378
  8. The Garlic Extracts Block the Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas Aeruginosa,R285
  9. Surveillance of Antimicrobial Resistance and Detection of Integron Gene Cassettes among Escherichia Coli Isolates from the Intestinal of Animals,S852.61
  10. Investigation and Diversity Analysis of the Antibiotic Resistant Integron-bearing Bacteria in Aquatic Environment,Q938
  11. Study on R-plasmid Curing Effect of Different Traditional Chinese Medicine to Three Kinds of Bacteria,S853.7
  12. Integrons Detected by Multiplex PCR and Their Effects on Antibiotic-resistance of Staphylococcus Aureus,R440
  13. Bacterial Resistance Detection and Analysis of Trands of Drug Resistance from Samples of Clinically Isolated,R446.5
  14. Research on Drug Resistant Genes of Plasmids in Multi-drug Resistant Acinetobacter Baumannii and Infective Chinese Herbals,R446.5
  15. Study on the Carbapenemases and Integrons among Carbapenem-resistant Acinetobacter Baumannii,R446.5
  16. Acinetobacter baumannii double ring of the aminoglycoside resistance phenomenon,R446.5
  17. Study of Drug-resistance Mechanism in Multi-drug Resistant Pseudomonas Aeruginosa,R446.5
  18. Study on Class Ⅰ Integron and ISCR1 in Non-Fermenting Gram-negative Bacilli from Clinical Specimens,R440
  19. Evaluation of Different Methods for Vibrio Parahaemolyticus Detection and Study on Characteristics of Genes in Environmental Strains,R155.5
  20. Production of 3-Hydroxypropionic Acid from Glycerol by Recombinant Klebsiella Pneumoniae,Q78
  21. The Construction and Clinical Application of DNA Microarray for Gram-negative Bacteria Drug Resistance Detection,R446.5

CLC: > Medicine, health > Clinical > Diagnostics > Laboratory diagnosis > Microbiological examination of
© 2012 www.DissertationTopic.Net  Mobile