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Analysis of ADV IgM Antibody and Clinical Character about Human Respiratary Tract Infection

Author: DongJiaZuo
Tutor: ZhangZhongLu
School: Dalian Medical University
Course: Department of Respiratory Medicine
Keywords: Adenovirus immunoadsorption experiment IgM antibody
CLC: R446.6
Type: Master's thesis
Year: 2013
Downloads: 13
Quote: 0
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Abstract


Aim:Use ELISA examine adult’s adenovirus, Describe and analyticalthe clinicalcharacteristic.Methods:Collect2012.2-2013.3Our hospital patient Serum that hospitalized and out-patient360case,The male is188, the female is172, the average age is48.2years old, and thesex proportion makes no difference. Each sufferer has lung CT or thoracic cavity slicecheck and grows disease diagnosis each time standard to see version7Medicineenzymology. The matched control is60, a healthy check-up crowd, the male is28, thefemale is32, the average age is42.3years old, and the sex proportion makes nodifference.Use ELISAExamine ADV3ADV7ADV11。ELISA examination method is to used for examinationto wrap drive at the solid phase epiphyseal plate bore in of treating and measuringantigen(or antibody), then use marking antibody, and adsorb given antigen or antibodythe surface is at the solid phase carrier and make antigen antibody respond to carry on inthe solid phase carrier surface and use to wash away dirt a method liquid mutually in ofvisiting and leaving the composition wash in addition to, finally pass Mao function toshow color to judge an effect after bottom thing.Results:123sufferers examine to bleed pure adenovirus IgM antibody in360impatient respiratory infections(ARI) sufferers to adult, among them, upper respiratory infections is79, and its infection adenovirus counts to32and checks a proptosis to lead40.5%. Theimpatient windpipe bronchitis is68, its infection adenovirus counts to27and checks aproptosis to lead39.7%.Pneumonia are78, its infection adenovirus counts to36and checks a proptosis to lead46.2%. The bronchietasis merges to be infected54, its infection adenovirus counts to8 and checks a proptosis to lead15%.The chronic obstruction lung disease acute aggravates81, its infection adenoviruscounts to20and checks a proptosis to lead25%.The matched control is60, theadenovirus is infected to count3, checking of adenovirus a rate and matched controladenovirus of adult impatient res piratory infections check a rate to have obviousdifference.(P<0.05)Concluison:1. Adenovirus3are one of the familiar pathogens of adult impatient res piratoryinfections(ARI)。2.Provide science basis for clinical treatment.3.Adenovirus in early days confirm can have already helped to hint whether it spreads,and carry on a prevention to different crowd.4. ELISA examination the method can provide technique support for the examination ofvirus and adopt immunoadsorption examination the method sensitivity is better, effectstability, price reasonable, suitable clinical of physically applied.

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