Dissertation > Excellent graduate degree dissertation topics show

New Methods for Detecting and Genotyping Hepatitis C Virus

Author: XiangDan
Tutor: ZhuHaiZhen
School: Hunan University
Course: Biomedical Engineering
Keywords: Molecular beacon HCV detection signal amplification gold nanoparticles
CLC: R512.63
Type: Master's thesis
Year: 2013
Downloads: 35
Quote: 0
Read: Download Dissertation

Abstract


Hepatitis C virus(HCV) has infected a population of over170million,while the vaccineof HCV is difficult to manufacture.What’s more,there is no specific medicine forit.Therefore,to diagnose HCV at an early time is definitly meaningful.At the presentstage,diagnostic methods for HCV are polymerase chain reaction(PCR) and enzyme-linkedimmunosorbent assay(ELISA).However,both of them are not convenient or time-savingenough.New convenient and sensitive methods are quite needful.Here we report some experiments based on molecular beacon(MB) and goldnanoparticles(AuNP) for directly detecting HCV RNA and genotyping of the virus.First partof this paper is using Duplex-specific nuclease(DSN) as a signal amplifying tool for MB todetect HCV RNA directly.In addition,we used MB to genotype HCV by detecting its PCRproduction.Moreover,AuNP are used to detect HCV RNA sensitively and easily.(1)While the hybridization of MB and RNA could be digested by DSN,the RNA wouldrecyle,which caused the signal being amplified.DSN could make MB2000times sensitivethan before.For JFH1RNA,standard of HCV RNA,we could detect it in a low concentration.And then,we detected the RNA of positive huh7.5cellline.We could distinguish positive andnegative serum RNA samples,the signals of which were basically corresponding to ELISA.(2) If we could use MB to detect the PCR production of RNA,gene orders of the emplatebeside the primer parts would be distinct.In this experiment,we fould the proper method andcondition for MB to detect double-stranded DNA(dsDNA),the PCR production.We designeda MB which targeted the different part of JFH1and H77S,subtypes of HCV,to distinguishthem.Hybridization of MB and the target part of dsDNA could easily distinguish differentgenotypes of virus RNA after their PCR.(3)In a saline solution,gold nanoparticles(AuNP) should adsorb lots of single-strandedDNA(ssDNA) to maintain the colloid state.If enough RNA could hybridize the ssDNA,AuNPwould just coagulate.Using primer,AuNP,saline solution,we could observe the color changingof AuNP(coagulation) or no changing while there was or not HCV RNA.What’s more,detecting the absorption on the UV-VIS spectrophotometer could make it more sensitive.Although not consummate yet,these methods are very simple,time-saving andspecific,which could be new mentalities for detecting HCV.

Related Dissertations

  1. A New Method for Thrombin Detection by Using Aptamers and Gold Nanoparticles,Q55
  2. Study on the Effect of Gold Nanoparticles in the Immunological Response Induced by Lipopolysaccharide,R318.08
  3. The Process of Cysteine Self-assembly on Gold Nanoparticle Modified Electrodes and Their Application,TB383.1
  4. The Application of Ordered Mesoporous Carbon and Glod Nanoparticles Composite in Bioensor,TB383.1
  5. Study on the Sensing Interfaces Fabrication of Electrochemical Impedance Immunosensor and the Application for the Detection of Polycyclic Aromatic Hydrocarbons,TP212.2
  6. Synthesis, Characterization and Biomedical Applications of Branched Polyethylenimine-and Dendrimer-Based Functionalized Nanomaterials,TB383.1
  7. Biosensor Based on Lipid Bilayers Modified by Au Nanoparticle for the Electrochemical Detection of Penicillin G Residues in Milk,TP212.3
  8. Dendrimer coated gold nanoparticles as non-viral vectors for gene transfer studies,R450
  9. Gold nanoparticles self-assembly behavior of,TB383.1
  10. Gold nanoparticles synthesis , surface modification and biological applications,TB383.1
  11. The Study of Preparation Electrochemical Properties and Surface Modification of Boron-doped Diamond Thin Film,TB383.2
  12. Preparation and Photo-catalytic Properties of the Visible Catalytic Materials Hybrid Gold Nanoparticles,X52
  13. Magnetic carbon nanotubes to build and microbial laccase fixed sensor technology research,Q813
  14. Application of Biofunctionalized Nanoparticles for Detection of Escherichia Coli O157:H7,R155
  15. Optical Biosensor Based on Organic Nano-functional Film,TB383.1
  16. Novel Luminescent Nanomaterials Labeling Based Detection Method for Salmonella,TB383
  17. Preparation of new bio- electrochemical sensors,TP212.2
  18. The Application of Micro/Nano Technology in DNA Detection and Antibody Purification,TB383.1
  19. New multi- color fluorescent nano- biological materials and research,TB383.1
  20. Design of Electrochemistry Immunosensor Based on Biocompatible Hybrid Nanomaterials,TB383.1
  21. Novel Fluorescence Detection of DNA Based on Gold Nanoparticles,O657.3

CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Viral Hepatitis > Hepatitis C (non- A non-B )
© 2012 www.DissertationTopic.Net  Mobile