Dissertation > Excellent graduate degree dissertation topics show

Regulation of the M2Anti-inflammatory Kupffer Cells by IL-4and CD4~+CD25~+FoxP3~+Regulatory T Cells on Severe Acute Pancreatitis in Mice

Author: DengCaiHong
Tutor: LiuJun
School: Huazhong University of Science and Technology
Course: Department of Gastroenterology,
Keywords: Severeacutepancreatitis IL-4 Treg Kuffercells M2pylorization.
CLC: R576
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation


Objective:To investigate the effective method of inducing severe acute pancreatitis with cerulein pluslipopolysaccharide (LPS) and the regulation of the M2anti-inflammatory kupffer cells by IL-4andCD4+CD25+FoxP3+regulatoryTcellsonsevereacutepancreatitisinmice.Methods:Normal group was induced by intraperitoneal injection of saline;model group was induced byintraperitonealinjectionofceruleinplusLPS.Modelgroupwasdividedintothreegroups:9h、12hand24hgroups after induction of SAP. Histopathological alterations of pancreatic tissues were evaluated amongthese three groups.2. Expressions of inflammatory cytokines mRNA in liver tissues were assessed byreal-timefluorescentquantitativereversetranscriptasepolymerasechainreaction(RT-PCR)betweennormalgroup and SAP8h+NS group. The mice of SAPmodels were divided into three groups in accordance withtheintravenousinjectionofthedifferentsolutions:SAPwithsalineinjectiongroup、SAPwithIL-4injectiongroup and SAPwith Treg injection group. Expression of IL-1β、TNF-α and IL-10mRNAin liver tissueswere assessed by RT-PCR;Expressions of CD163and CCR7in liver were assessed by confocalimmunofluorescencemicroscopy.Results:(1)TheresultsofHEstaining:pancreaticedema、inflammationandacinarcellnecrosisin24hgroupsafterinductionofSAP.(2)TheexpressionsofIL-1βandTNF-αmRNAinlivertissuesofSAP8h+NSgroupweresignificantlyhigherthanthoseofnormalgroup(P<0.1).(3)TheexpressionsofIL-1βmRNAinlivertissuesofSAP16h+TreggroupandSAP16h+IL-4groupweresignificantlylowerthanthoseofSAP16h+NSgroup(P<0.1));TheexpressionofIL-1βmRNAinlivertissuesofSAP16h+TreggroupwassignificantlylowerthanthoseofSAP16h+IL-4group(P<0.05);TheexpressionsofTNF-αmRNAinlivertissuesofSAP16h+TreggroupandSAP16h+IL-4group weresignificantlylowerthanthoseofSAP16h+NSgroup((P<0.1);TheexpressionofTNF-αmRNAinlivertissuesofSAP16h+TreggroupwassignificantlylowerthanthoseofSAP16h+IL-4group(P<0.1);TheexpressionofIL-10mRNAinlivertissuesofSAP16h+IL-4group wassignificantlyhigherthanthoseofSAP16h+NSgroupand SAP16h+Treggroup(P<0.1).Conclusion:It is a effective method using cerulein plus LPS to induce severe acute pancreatitis in mice. Kuffer cells arevery important in the development of SAP. IL-4and Treg promote the expression of the M2anti-inflammatorykupffercells,andthedown-regulationofTNF-α、IL-1βandCCR7andup-regulationofIL-10andCD163suggestIL-4andTreghavetherapeuticeffectsonSAP.

Related Dissertations

  1. Inhibition of RAW264.7 Macrophage Inflammatory Cytokines Release by Guggulsterone,R285
  2. Infectious Bursal Disease Virus Particle Vaccine Research and Its Immune Efficacy Evaluation,S858.31
  3. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  4. The Expression and Significance of Nuclear Factor Kappa B (NF-κb) and IL-6 in the Degenerated Cervical Intervertebral Disc,R681.55
  5. Cysteamine on T Lymphocyte Proliferation in Post-Operation Goats and Its Mechanism Underlying,S858.27
  6. Cloning, Expression and Bioactivity Analysis of Goat Interleukin-1β and Interleukin-6,S827
  7. Meridians of Asthma Thread Burying Model Rats IL-4 Such Impact Experimental Study,R245
  8. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  9. Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats,R285.5
  10. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  11. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  12. Clinical Observation of Ditan Soup in Treating PSD,R277.7
  13. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  14. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  15. The Significance and the Expression of the Levels of IL-13 and Eosinophil Count and IgE in Asthma Children Infected with Mycoplasma,R725.6
  16. Septic lung injury (ALI / ARDS) in patients with peripheral immune parameters Th1, Th2, HLA-DR and Treg changes and Integrative Medicine,R459.7
  17. IL-6, TNF-α in animal models of postoperative ileus expression and security organs of the protective effect of Chinese medicine research,R285.5
  18. PNS treatment of rabbit lung contusion in rats,R285.5
  19. Asthma serum IL-17 and IgE changes and Correlation Analysis,R562.25
  20. Glucocorticoid treatment of bronchial asthma patients serum IL-18, IL-33 levels in,R562.25
  21. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2

CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Pancreatic diseases
© 2012 www.DissertationTopic.Net  Mobile