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Long Non-coding RNA MALAT-1and Zinc Finger Protein Transcription Factor Snail Expression in Renal Cell Carcinoma and Their Correlation Study

Author: GuTingTing
Tutor: ZhangAiLi
School: Hebei Medical University
Course: Surgery
Keywords: renal cell carcinoma (RCC) MALAT-1 Snail longnon-coding RNAs Reverse transcription polymerase chain reaction (RT-PCR)
CLC: R737.11
Type: Master's thesis
Year: 2014
Downloads: 9
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Abstract


Objective: Renal cell carcinoma(RCC) is one of the common malignanttumors in the genito-urinary system. It is originated in the renal urinary tubularepithelial system which is one of the most common malignant tumor of kidneyaccounting for about2%to3%of adult malignancies and80%~90%[1]primary malignant tumor. The unconspicuous early symptoms of renal cellcarcinoma and non-specific features make the incidence of renal cellcarcinoma ascended in recent year. At present, the molecular mechanism ofoccurrence and development of renal cell carcinoma is not clear and theradiotherapy and chemotherapy of renal cell carcinoma is ineffective. Radicalresection operation is considered as the most effective treatment, but nearly30%of patients with limit renal cell carcinoma appear tumor recurrence aftertreatments[2]. The recurrence and metastasis of tumor is still the main factorsthreatening the lives of patients. At present, in addition to imaging results, thelack of early laboratory diagnostic indicators make early detection, earlydiagnosis and treatment a difficulty issue. In order to improve the treatment ofpatients with renal cell carcinoma results, seeking new biomarkers of earlydiagnosis and prognosis and the futher study on its important role in theoccurrence and development of renal cell carcinoma have an importantsignificance.The function of long non-coding RNA in the tumor’s occurrence andtumor’s development has been more and more obvious, which can regulate theexpression of genes in multiple levels,involving the growth, invasion andmetastasis in tumor. The abnormal expression of the MALAT-1in humantumor tissues change the biological phenotype of tumor cells, increase theproliferation ability of tumor cells and improve the metastasis ability and invasion ability. To promote the occurrence and development of tumor. It isfound that MALAT-1is regulated by the upstream transcription factor ofSnail,Sox-5,FOXQ1and RUNX1through predicting and screening for breastcancer. In the meanwhile, MALAT-1can regulate the expression ofdownstream transcription factor of hsa-miR-320a. So MALAT-1and Snailtogether have an important role as "cancer promoting gene" in the occurrenceand development of renal cell carcinoma. This thesis about renal cellcarcinoma is going to detect the expression of MALAT-1and its upstreamtranscription factor Snail in fresh isolated kidney cancer and correspondingnormal tissues, to explore the relationship between MALAT-1and renal cellcarcinoma, as well as the wrelation between Snail and renal cell carcinoma,the same as connection between MALAT-1, and Snail and their possiblemechanism of action.Method:Reverse transcription polymerase chain reaction (RT-PCR) method wasused to detect the mRNA level of long non-coding RNAs MALAT-1and itsupstream transcription factor Snail gene in58RCC tumor tissues andcorresponding normal tissues, demonstrated that relationship between RCCtumor and corresponding normal tissues individual expression, so does therelationship between MALAT-1and Snail.Result:1.1The mRNA relative expression of MALAT-1in RCC tissuesMALAT-1mRNA relative expression in tumor tissues (1.08±0.26) wassignificantly higher than that in corresponding normal tissues (0.84±0.27)(P<0.05). The mRNA relative expression of MALAT-1was not associatedwith status of gender(male and female), age(<60and≥60), the maximumdiameter of tumor (≤7cm and>7cm)and TNM stage(Ⅰ+Ⅱ andⅢ+Ⅳ) ofRCC patients (P <0.05).1.2The mRNA relative expression of Snail in RCC tissuesSnail mRNA relative expression in tumor tissues (0.83±0.26) was significantlyhigher than that in corresponding normal tissues (0.61±0.33)(P<0.05). the mRNA relative expression of Snail was not associated with status ofgender(male and female), age(<60and≥60), the maximum diameter of tumor(≤7cm and>7cm)and TNM stage(Ⅰ+Ⅱ andⅢ+Ⅳ) of RCC patients (P<0.05).2The relationship between the mRNA relative expression of MALAT-1and mRNA relative expression of Snail in human renal cell carcinoma(RCC)Through the test of normality respectively, the relative expression ofMALAT-1mRNA and Snail mRNA in human renal cell carcinoma conform tonormal distribution. Then, through statistical methods of linear correlation, therelative expression of MALAT-1mRNA and Snail mRNA in human renal cellcarcinoma conform to positive correlation (r=0.396, P=0.003<0.01).Conclusions:1MALAT-1mRNA relative expression in RCC tissues was significantlyhigher than that in corresponding normal tissues, It Prompts that there is acorrelation between abnormal expression of MALAT-1and the occurrence anddevelopment of tumor. It was not associated with status of gender, age, themaximum diameter of tumor and TNM stage of factors.2Snail mRNA relative expression in RCC tissues was significantly higherthan that in corresponding normal tissues, It Prompts that there is a correlationbetween abnormal expression of MALAT-1and the occurrence anddevelopment of tumor. It was not associated with status of gender, age, themaximum diameter of tumor and TNM stage of factors.3Through this study, we found that the relative expression levels ofMALAT-1and Snail in renal cell carcinoma are significantly increased.Besides, there is a positive correlation between MALAT-1and Snail. As one ofthe upstream transcription factor of MALAT-1, Snail can regulate theMALAT-1gene and promote the occurrence and development of tumor.Conclusively, the Snail gene will become a new target for diagnosis andtargeted therapy in renal cell carcinoma.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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