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Fibroblast Growth Factor-2Counteracts the Effect of Ciliary Neurotrophic Factor on Spontaneous Differentiation in Adult Hippocampal Progenitor Cells

Author: HeZhiLi
Tutor: SunShengGang
School: Huazhong University of Science and Technology
Course: Department of Neurology
Keywords: spontaneous differentiation neural progenitor cells basic fibroblastgrowth factor neurogenesis
CLC: R741
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
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Neural stem/progenitor cells (NSCs) can spontaneously differentiate into neurons andglial cells in the absence of mitogen fibroblast growth factor-2(FGF-2) or epidermalgrowth factor (EGF) in medium and the spontaneous differentiation of NSCs ismediated partially by endogenous ciliary neurotrophic factor (CNTF). This studyexamined the relationship of FGF-2and CNTF in the spontaneous differentiation ofadult hippocampal progenitor cells (AHPs). AHPs were cultured in the medium con-taining different concentration of FGF-2(1–100ng/mL). Western blotting andimmunofluorescence staining were applied to detect the expression of the astrocyticmarker GFAP, the neuronal marker Tuj1, the oligodendrocytic marker CNPase and,Nestin, the marker of AHPs. The expression of endogenous CNTF in AHPs at early(passage4) and late stage (passage22) was also measured by Western blotting. Theresults showed that FGF-2increased the expression of Nestin, dramatically inhibitedthe expression of GFAP and Tuj1and slightly suppressed the expression of CNPase.FGF-2down-regulated the expression of endogenous CNTF in AHPs at both early(passage4) and late stage (passage22). These results suggested that FGF-2couldinhibit the spontaneous differentiation of cultured AHPs by negatively regulating theexpression of endogenous CNTF in AHPs.

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