Dissertation > Excellent graduate degree dissertation topics show

Fibroblast Growth Factor-2Counteracts the Effect of Ciliary Neurotrophic Factor on Spontaneous Differentiation in Adult Hippocampal Progenitor Cells

Author: HeZhiLi
Tutor: SunShengGang
School: Huazhong University of Science and Technology
Course: Department of Neurology
Keywords: spontaneous differentiation neural progenitor cells basic fibroblastgrowth factor neurogenesis
CLC: R741
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation

Abstract


Neural stem/progenitor cells (NSCs) can spontaneously differentiate into neurons andglial cells in the absence of mitogen fibroblast growth factor-2(FGF-2) or epidermalgrowth factor (EGF) in medium and the spontaneous differentiation of NSCs ismediated partially by endogenous ciliary neurotrophic factor (CNTF). This studyexamined the relationship of FGF-2and CNTF in the spontaneous differentiation ofadult hippocampal progenitor cells (AHPs). AHPs were cultured in the medium con-taining different concentration of FGF-2(1–100ng/mL). Western blotting andimmunofluorescence staining were applied to detect the expression of the astrocyticmarker GFAP, the neuronal marker Tuj1, the oligodendrocytic marker CNPase and,Nestin, the marker of AHPs. The expression of endogenous CNTF in AHPs at early(passage4) and late stage (passage22) was also measured by Western blotting. Theresults showed that FGF-2increased the expression of Nestin, dramatically inhibitedthe expression of GFAP and Tuj1and slightly suppressed the expression of CNPase.FGF-2down-regulated the expression of endogenous CNTF in AHPs at both early(passage4) and late stage (passage22). These results suggested that FGF-2couldinhibit the spontaneous differentiation of cultured AHPs by negatively regulating theexpression of endogenous CNTF in AHPs.

Related Dissertations

  1. Effects of Erythropoietin on Improving White Matter Injury and Enhancing Neurogenesis in Newborn Rats with Hypoxia-Ischemic Brain Damage,R722.19
  2. Effects of Ca2+ Handing Proteins on Endoplasmic Reticulum Ca2+ Content and Neuronal Differentiation in Mouse Embryonic Stem Cells,R329
  3. Original cadherin (Protocadherin) 18b in zebrafish embryos role in nervous system development,Q75
  4. Long-term Effect of Stroke on the Neurogenesis of Dentate Gyrus in the Adult Rats,R743.3
  5. Expression Patterns of Ndrg2 in Developing and Adult Mouse Brain,R346
  6. Environmental enrichment improved spatial learning after cerebral ischemia and its mechanism,R743.3
  7. Study on the Effects of Cognitive Function Caused by Hypoxic Injury in the Neonate Rats,R766
  8. Effect of 5-HT1A Receptor Activator 8-0H-DPAT on Neural Plasticity in Epilepric Rats with Depression,R742.1
  9. The Effect of Liquid Petroleum Gas Poisoning on Neurogenesis and Learning and Memory in Mice, and the Intervention of BFGF,R595.1
  10. Neurosteroids hormone pregnenolone sulfide and DHEA regeneration of the AD brain,Q42
  11. Cytokines in the pathological effects of stress-induced depression,R749.4
  12. Effects of LBP Treat AD Mice on Hippocampal Dentate Gyrus Neurogenesis and Microglia,R285
  13. Noggin by upregulating the expression of VEGF in rat striatum after cerebral ischemia and neuronal regeneration,R743.3
  14. Effects of Ginsinoside Rd on Neurogenesis in the Dentate Gyrus of Normal and Infrasound-exposed Adult Rats,R285.5
  15. Improve cerebral lymphatic drainage pathways of the role of prevention in acute cerebral infarction,R743.3
  16. Effects of Erythropoietin on the Neurogenesis and Cognitive Function of Vascular Dementia Rats,R749.13
  17. Effects of Polygala Tenuifolia Willd Treat AD Mice on Hippocampal Dentate Gyrus Neurogenesis and Microglia,R285.5
  18. Research on Cholinergic Neural Cells Derived from Mouse ES Cells and Mitochondria-related Neurogensis Mechanisms Initiated by Icaritin,R285.5
  19. Role of PEDF in Neuroblastic Differentiation and Survival Analysis Combined with Clinical Data,R73-3
  20. Learning and Running Enhance Neurogenesis in the Hippocampal Dentate Gyrus of Adult Rats and Improve Spatial Learning Ability,Q426
  21. The Experimental Study of Neurogenesis and Correlation between Neurogenesis and Vascular Endothelial Cell Growth Factor after Focal Cerebral Ischemia in the Rat,R743

CLC: > Medicine, health > Neurology and psychiatry > Neurology
© 2012 www.DissertationTopic.Net  Mobile