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Identification of the Target Genes of Transcription Factor Cebpb in Acute Promyelocytic Leukemia Cells Induced by All-trans Retinoic Acid(Application of Synthetic Biology in Medicine)

Author: YuLei
Tutor: ZhangYangDe
School: Central South University
Course: Biomedical Engineering
Keywords: chromatin immunoprecipitation(ChIP) all-trans retinoicacid CEBP granulopoiesis neutrophils
CLC: R733.71
Type: PhD thesis
Year: 2013
Downloads: 44
Quote: 0
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Abstract


This study is to explore the mechanism of transcription factor CEBPB (CCAAT enhancer-binding protein β) in regulation of the acute promyelocytic leukemia (APL) cell differentiation induced by All-trans retinoic acid (ATRA). The method of combining chromatin immunoprecipitation (ChIP) with in vitro selection was applied for high-throughput identification of the CEBPB downstream direct target genes. One Hundred and six CEBPB binding fragments from the genome of the ATRA-treated NB4cells were selected after ChIP. Among them,82were mapped in proximity to the known or previously predicted genes; meanwhile,7were randomly picked up for further confirmation by ChIP-PCR and finally3genes (GALM, ITPR2and ORM2) were found to be specifically up-regulated in ATRA-treated NB4cells, indicating that they might be the down-stream target genes of transcription factor CEBPB. The results provide a new insight into the mechanisms of ATRA-induced granulocytic differentiation.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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