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Objective:To investigate the influence of deguelin, a rotenoid from Mundulea sericea, on the proliferation,cell cycle,apoptosis and autophage of Panc-1cell and preliminary explore the underlying mechanisms of autophage induced by deguelin.Methods:CCK-8assay detected the cell proliferation; PI staining was used to observe the change of cell cycle;Annexin V-FITC assay was performed to test apoptosisl; In order to examine the autophage, monodansylcadaverine (MDC) and acridine orange/ethidium bromide (AO/EO) stain was adopted; Immunoblotting (Western Blot) explored the expression of proteins include cell cycle, apoptosis, autophage, Akt/mTOR and MAPK signal.Results:After the intervention of24h,48h and72hours, the survival of Panc-1cells was significantly decreased with a drug concentration and time-dependent manner (P<0.05); After the intervention of24h and48h hours, G2/M phase was significantly increased and cell cycle proteins cylinA,cylinB,cdk2, p-cdc25and p21expression was increased, cylinD1, cylinD3, cdk4and cdk6expression was decreased; After48hours of drug intervention,the low concentration of deguelin does not promote Panc-1cell apoptosis (P>0.05), the depth of deguelin(100μM,200μM) can induce the PANC-1cell apoptosis (P<0.05); The staining of MDC within the point-like positive structure and AO/EO within the orange-red fluorescence significantly increased;Atg5, Atg7and LC3Ⅱ expression increased obviously; p-Akt and p-mTOR expression is reduced, p-Erk, p-p38and p-Jnk expression is ncreased.Conclusions:Deguelin inhibits the proliferation and induces autophage of Panc-1cell significantly. Deguelin also affects the expression of cell cycle and autophage proteins. Deguelin induces the autophage through the activation of autophagy proteins, Akt/mTOR and MAPK signal involved in the regulation of autophage.
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