Dissertation > Excellent graduate degree dissertation topics show

Study on the Autophagy Related Gene after Focal Cerebral Ischemia/reperfusion and the Effects of Artificial Synthetic E-selectin in Rats

Author: YangJun
Tutor: ZhangShiMing
School: Suzhou University
Course: Neurosurgery
Keywords: autophagy cerebral ischemia reperfusion injury Beclin l PTEN artificial synthetic E-selectin
CLC: R651.1
Type: Master's thesis
Year: 2011
Downloads: 126
Quote: 0
Read: Download Dissertation

Abstract


Objective:To investigate the changes of autophagy and lysosome after focal cerebral ischemia/reperfusion in rats and approach the effects of artificial synthetic E-selectin of autophagy/lysosomal pathway in brain injury after focal cerebral ischemia/reperfusion.Methods: Healthy Sprague—Dawley rats were randomly divided into operation group,sham—operation group,artificial synthetic E—selectin treated group. The formation of autophagosome and activation of lysosome were observed in neuron after cerebral ischemia/reperfusion by using transmission electron microscope (TEM),Immunohistochemistry was employed to determine the expression of Beclin l and PTEN in ischemic penumbra (cortex). Artificial synthetic E-selectin was used to interfere autophagy pathway before cerebral ischemia, its effects were analyzed to find its effects on autophagy.Results:There were few number of autophagosomes and lysosomes in neuron of sham-operated group.The formation of autophagosomes and enhanced presence of lysosomes were detected by TEM at 4h in ischemic cortex after cerebral ischemia/ reperfusion,its number was significantly increased at 24h,and still could be found at 5d. There was little expression of Beclin 1 and PTEN in neuron of sham-operated group. The expression of Beclin 1 and PTEN detected by immunohistochemistry was significantly increased at 4h (P<0.01) and peaked at 24h after cerebral ischemia/reperfusion,its expression still could be found at 5d (P<0.01). The varied trendcy of Beclin 1 and PTEN in artificial synthetic E-selectin treated group was the same as operation group,but increased much obvious at each time point(P<0.05).Conclusions:1.There were few number of autophagosomes,low expression of Beclin 1 and PTEN were detected in normal neuron of rats;2. Autophagy/lysosomal pathway was activated after focal cerebral ischemia/reperfusion in rats.Its activation was manifested by the formation of autophagosomes,the activation of lysosomes and the robust elevation of Beclin 1,PTEN in ischemic penumbra with the time challenged by cerebral ischemia/reperfusion injury;3. Artificial synthetic E-selectin has protective effects on cerebral ischemia/reperfusion injury in rats. Activation of autophagy might play a protective role in the initial stage of cerebral ischemia/reperfusion.

Related Dissertations

  1. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  2. The Relevance Research of Neuroglobin and Caspase-3 in the Hippocampus CA1 Regin in Global Cerebral Ischemia-reperfused Rats,R741
  3. Neuroprotective Mechanism of NBP Preconditioning in Cerebral Ischemia Reperfusion Injury on Sprague-Dawley Rats,R743.33
  4. Research of Expression and Clinical Significance of p53、PCNA and PTEN in Gastric Cancer,R735.2
  5. Exogenous PCr anti- animal brain ischemia-reperfusion injury and brain-derived experimental study MODS,R965
  6. survivin and PTEN in epithelial ovarian tumors and its significance,R737.31
  7. Effect of Different Glucose Concentrations on Autophagy in Neonatal Rat Cardiomyocyte Culture,R542.2
  8. Apoptosis Mechanisms Research in Interfering TIGAR Gene and Epirubicin Treatmnet in Hepatic Carcinoma Cells,R735.7
  9. The Effect of Gefitinib in Radiosensitization Through Autophagy Regulation in Human Non-small-cell Lung Cancer A549 Cell Line,R734.2
  10. Amiloride Is Protective in PC12 Cells Via Autophagy Pathway,R329
  11. The Effects of ER Stress on Cell Toxicity and Htt Levels in the Stable PC12 Cells Expressing Htt586,R363
  12. Intervention and Mechanisms of E-64d on Developmental Seizure-induced Cognitive Deficit,R720.597
  13. Effects of Lysosomal Enzyme Inhibitor on Recurrent Neonatal Seizure Induced Acute and Long-term Gene Expression and Behavioral Changes,R720.597
  14. Effects of the Artificial Synthetic E-selectin on the Expression of NF-κB and MCP-1 in an Elastase-induced Model of Common Carotid Artery Aneurysm in Rabbits,R651.1
  15. The Effects of Autophagy on Vascular Smooth Muscle Cells Proliferation,R363
  16. Effects of siRNA Inhibit Class I PI3K on Humangastric Cancer SGC7901 Cells,R735.2
  17. Effects of Combined Therapy of 5-FU and Inhibitor of ClassⅢ PI3K Human Gastric Cancer SGC7901 Cells,R735.2
  18. Effects of Mutant Huntingtin-552 on Glutamate Transporter GLT-1 in Astrocytes Are Reversed by Rapamycin,R742.2
  19. Mechanisms of EGCG Attenuating Focal Cerebral Ischemia and Reperfusion Injury in Rats,R285.5
  20. The Microcirculation Dysfunction and the Expression of Fgl2 in the Rat Model of Acute Cerebral Ischemia/reperfusion,R743
  21. The Effects of Autophagy on Cardiomyocytes Viability and Mitochondrial Membrane Potential in Sepsis,R459.7

CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Brain
© 2012 www.DissertationTopic.Net  Mobile