Dissertation > Excellent graduate degree dissertation topics show

Nicorandil Alleviates the Oxidative Stress in Endothelial Cells by Regulating the Thioredoxin Expression

Author: LiYuanYuan
Tutor: QiBenLing
School: Huazhong University of Science and Technology
Course: Geriatrics
Keywords: Cell model Human umbilical vein endothelial cells (HUVECs) Peroxide hydrogen （H2O2） Oxidative stressNicorandil Thioredoxin Human umbilical vein endothelial cells(HUVECs) Reactive oxygen species(ROS) ATP-sensitive K+channels Glibenclamide
CLC: R96
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation

Abstract


PartⅠ Establishment and evaluation of the H2O2-inducedoxidative stress model in HUVECsObjective: To establish and evaluate the cell model of H2O2-induced oxidativestress in HUVECs.Methods: HUVECs were divided into6groups. HUVECs were exposed to50μmol/L H2O2，100μmol/LH2O2，200μmol/LH2O2，400μmol/LH2O2for6hours，12hours，24hours. After incubation, HUVECs were washed, placed inphenol-red-free and serum-free DMEM. Intracellular ROS were visualized by usingan inverted fluorescence microscope （Leica RX-DA），cells were treated with theabove dye （DCFH-DA in HBSS） and were measured by FACS analysis，fluorescence intensity of the total culture was used as an indicator.Results: H2O2exposurment increased ROS formation in HUVECs， differentconcentrations along with different times induced different ROS formation inHUVECs. Treatment with100μmol/L H2O2for24h markedly increased intracellularROS levels in HUVECs under static culture conditions. And then, intracellular ROSlevels were decreased and the apoptosis of HUVECs were increased.Conclusion: Treatment with100μmol/L H2O2for24hours markedly increasedintracellular ROS levels in HUVECs under static culture conditions, and the cellmodel of oxidative stress in HUVECs were established. PartⅡ Nicorandil alleviates the oxidative stress in endothelialcells by regulating the thioredoxin expressionObjective: The purpose of this study was to estimate the influence of nicorandilin human umbilical vein endothelial cells oxidative stress induced by H2O2and theexpression of thioredoxin–one of the antioxidant systems in cells.Methods: Human umbilical vein endothelial cells were randomly allocated intofour groups:①control group;②oxidative stress cell model group;③oxidativestress cell model group with nicorandil;④oxidative stress cell model group withnicorandil as well as a KATPchannel blocker glibenclamide. HUVECs weremaintained in a humidified5%CO2in air incubator at37℃. Oxidative stress wasassessed by the formation of ROS through Flow Cytometry（FCM）. ThioredoxinmRNA levels were measured by real-time PCR, thioredoxin expression was analyzedby western blot.Results: Nicorandil treatment significantly attenuated ROS formation of humanumbilical vein endothelial cells induced by H2O2, up-regulated the mRNA levels ofthioredoxin, and elevated the production of thioredoxin protein. Furthermore, theKATPchannel blocker glibenclamide abolished nicorandil-mediated reduction ofreactive oxygen species as well as mRNA level of thioredoxin.Conclusions: The present study has revealed that nicorandil attenuatesH2O2-induced ROS formation in human umbilical vein endothelial cells throughincreased the production of thioredoxin.

Related Dissertations

  1. Functional Analysis of ACD5 during Infection of Botrytis Cinerea in Arabidopsis Thaliana,S432.1
  2. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  3. Effects of K ATP -channel Inhibition Glibenclamide on Lung Injury in a Rat Model of Hemorrhagic Shock,R605.971
  4. RNAi Down-regulated Thioredoxin Expression Sensitizes U251 Cells to Arsenic Trioxide-induced Apoptosis,R739.4
  5. OGRE Engine Based on Three-dimensional Modeling of Biological Cells Research and Application,TP391.41
  6. The Association of TXNIP with Type 2 Diabetes and Insulin Resistance,R587.1
  7. Effects of Different Temperatures on Redox Status in Pupae of Orthaga Achatina Butler,S763.7
  8. Modelling and Design of Multitubular Fixed Bed Reactors,TQ052
  9. Comparative Study on the Intestinal Absorption Mechanism of Berberine and 8-hydroxy Dihydroberberine,R96
  10. Modeling of Photovoltaic Cells and Its Application in Energy Prediction,TM914.4
  11. Expression and Immunogenicity Analysis of Thioredoxin Reductase of Vibrio Harveyi SF1 on Turbot (Scophthalmus Maximus),S943
  12. The Effect and Mechanism of 15 - Epimer ╟lipoxin A4 Reduce Reactive Oxygen Species Generation in Bv-2 Microglia Induced by Lipopolysaccharide,R363
  13. Impact of High Glucose on the Vascular Endothelial Cell Apoptosis and the Protective Effect of NO,R587.2
  14. Three-dimensional angle interlock woven composites mechanical properties of low-velocity impact and Finite Element Analysis,TB332
  15. The levocarnitine cardioprotective effect of ATP-sensitive potassium channels in the relationship,R965
  16. Design and Implement of GIS Math Model Base System Based on Meta Algorithm,P208
  17. The Function of O1 Type Thioredoxin Gene in Arabidopsis Thaliana under Environmental Stress,Q945.78
  18. Study of Stand-Alone Photoltaic System for CAP Lamp Charging,TM615
  19. Morphine Affected Thioredoxin Expression in Mice Brain,R96
  20. The Relationship between TRX-binding Protein-2 and Breast Cancer,R737.9
  21. Related Research on Morphine and Thioredoxin,R749.6

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile