Dissertation > Excellent graduate degree dissertation topics show

The Effection of Estrogen on Adipogenic Differentiation of Kidney Fat Capsule Adipose-derived Mesenchymal Stem Cells Which Were after Long-term Cryopreservation

Author: ZhangHui
Tutor: ZhengHongGuang
School: Liaoning Medical
Course: Internal Medicine
Keywords: estrogen long-term cryopreservation kidney fat capsule adipose-derivedstem cells adipogenic differentiation
CLC: R96
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo explore the impact of estrogen on adipogenic differentiation of long-termcryopreservation and the fresh kidney fat capsule adipose-derived mesenchymalstem cells(KFC-ADSCs).MethodsThe third generation of long-term cryopreserved and the fresh KFC-ADSCswere divided into four groups, and all of which were induced to adipogenic cellsby induced fluid，which content dexamethasone, insulin, indomethacin,isobutylmethylxanthine. Among them,the long-term cryopreserved KFC-ADSCs wasdivided into group A[given low-dose estrogen (10-7M))] and group B(only giventhe induced fluid).The fresh KFC-ADSCs were divided into group C and group D,group C was given low-dose estrogen (10-7M) and induced fluid to induceadipogenic differentiation, group D was only given to induce adipogenicdifferentiation the induced fluid.ResultsThe morphology of the third generation of long-term cryopreserved and freshKFC-ADSCs both were fusiform, polygonal, showing whirlpool arragement, therewas no significant difference between the third generation of long-termcryopreserved and fresh KFC-ADSCs in morphology and cells arragement; Cell Surface antigen CD29, CD44were all detected positiveexpression, but CD31wasall detected negative expression; Intracellular lipid droplets in the cells wereobserved after adipogenic differentiation by oil red O staining, the cells all showedpositive oil red O staining; The Adipogenic volume comparison of each groupafter adipogenic differentiation was detected in the fourteenth day [TheAbsorbance value(value A)which was measured by spectrophotometer wasselected as comparative target].Compared with the group A, the Absorbancevalue of the group B was significant higher(P<0.05);compared with the group C,the Absorbance value of the group D was significant higher(P<0.05). But nosignificant difference was detected in group A and group C (P>0.05).ConclusionsLow-dose estrogen can inhibit the adipogenic differentiation of long-termcryopreservation KFC-ADSCs. There was no significant difference between thethird generations of long-term cryopreserved and the fresh KFC-ADSCs onadipogenic differentiation.

Related Dissertations

  1. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  2. Identification of Recombinant Adenovirus Carrying Mouse Estrogen Receptor α in Infected Neurons and Central Nervous System,R363
  3. The Correlation of Ultrasound Elasticity with the Expressions of Estrogen Receptor, Progestron Receptor, C-erbB-2 in Breast Cancer,R445.1
  4. Influence of Estrogen and H.pylori on Human Duodenal Mucosal Bicarbonate Transport Protein,R573.1
  5. Differentiation Capacity of Periodontal Ligament Stem Cells of Chronic Periodontitis Patients with and Without Type 2 Diabetes Mellitus,R587.1
  6. Expressions of HOXA9 HOXA10 and HOXA11 in Breast Cancer,R737.9
  7. Effects of Estrogen on Proliferation and Differentiation of Human Embryo Mandibular Condylar Chondrocytes,R782.6
  8. Effect of Advanced Glycation End Products (AGEs) on Biological Characteristics of Lipopolysaccharide (LPS)-mediated Periodontal Ligament Stem Cells,R781.4
  9. Estrogen Sulfate Transferase (SULT1E1) Expression in Breast Cancer Tissues and Its Clinical Significance,R737.9
  10. Separation of Phenolic Enviromental Endocrine in Wastewater on a PDMS Microchip,X832
  11. The Impact of E2 on Thyroid Cells Proliferation and Apoptosis,R736.1
  12. Ginsenoside Rg1 Protects Against β Amyloid-Induced Neurotoxicity Via Activation of Er and IGF-IR Signaling Pathways,R285.5
  13. The Effect of Estrogen and 1, 2-dimethylhydrazine on Crypt Fission of Rat Colon,R735.35
  14. Study on Edcs Removal and Microorganism in Soil Wastewater Treatment System,X703
  15. Research on Removal of Typical Environmental Estrogens in Aqueous Solution by Dielectric Barrier Discharge Over Water Process,X703
  16. Association of Polymorphisms in Estrogen Receptors with the Risk of Colorectal Cancer,R735.34
  17. Exploration of High-risk Factors of Ectopic Pregnancy in Vitro Fertilization and Embryo Transfer,R714.8
  18. Association of Estrogen and Heat Shock Protein 27 to Coronary Heart Disease and Their Effects on Endothelial Cell Function,R541.4
  19. Study on Extract Prepared of Kudzu Vine and Its Effect on Resistance Osteoporosis,R285.5
  20. Effects of Gold Nanoparticles and Carbon Nanotubes on Osteogenic and Adipogenic Differentiation of Bone Marrow Stromal Cells,TB383.1
  21. Mitochondrial Dysfunction in the Multiplicity Rodent Model for Alzheimer’s Disease Induced by Long-term D-galactose Injection Combined with Ovariectomy,R749.16

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile