Dissertation > Excellent graduate degree dissertation topics show

Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut

Author: LiZuo
Tutor: CaoHui
School: Nanjing Agricultural College
Course: Ecology
Keywords: DGGE RFLP Peanut Continuous Cropping Bacillus Photosynthetic Bacteria Microbial diversity
CLC: S565.2
Type: Master's thesis
Year: 2011
Downloads: 27
Quote: 0
Read: Download Dissertation

Abstract


Peanuts are the main oil crops and economic crops in low-hillt red soil region of south China. Culture without rotation in the peanut yield, the output and the VCR are tending to decline in 2-5 years. Some researchs from different angles analysed the reasons of continuous cropping obstacles, showing unbalance of soil microbial population was the most important elementary of causing crop. Based on extracting the total community DNA by the direct lysis method, Bacterial universal primers were used to amplify 16S rDNA V3 region fragments and fungal universal primers were used to amplify 18S rDNA of fungus; DGGE method was applied to study microbial community structure in different samples. By the method of isolating pure Bacullus strains from soil samples, Bacterial universal primers were used to amplify 16S rDNA fragments; PCR products digested by restriction endonucleases Hha I and Xsp I, and their fingerprints analyzed for diversity of Bacullus species from soils.The result of DGGE about Bacterial showed that band numbers were abundant in all the soil samples, revealed that richness of bacteria in three soils was relatively high. Dominant species mainly distribution in Bacullus. While the result of DGGE about fungus showed that band numbers were few in all the soil samples, revealed that richness of bacteria in three soils was not high. Mould were detected widespread in peanut continuous cropping soils. Cluster Analysis showed that almost same soil type samples were in same Cluster.Bacillus subtilis have more reports in the studies of Bacillus, widely existing in soil, lakes, oceans and surface plants and animals. Not only because of their non-pathogenic and can secrete a variety of enzymes and antibiotics but also have a good foundation of Fermentation, so they have a very wide range of uses. The result showed that the total number of culturable Bacullus spp.were high, being 2.00×105~2.25×105cfu/g dry soil. The three gene libraries included 9 OUTs restriction endonuclease types. Diversity index of PCC 1 were higher than other two samples, which suggested Bacullus spp. were more abundant in PCC 1. There were three main restriction types accounting for 54%、12% and 14% of the total 16S rDNA clones, respectively. They were B. pumilus, B. subtilis and B. altitudinis.In order to alleviate peanuts continuous cropping obstacles, the application of microbial agents were used to the soils of peanut continuous cropping obstacles, observing the peanut fertility quality and yield of influence in different treatments. The results showed: the microorganism preparation could increase the crops the output to a certain extent, which is increased by 32.14% compared to the control. As a microorganisms regulator, Bacillus are new environmentally friendly bio-agents to anti-cropping, expected to become an economic and effective countermeasure to reduce or lift the barriers of peanut cropping in the future.

Related Dissertations

  1. Study on the System of Continous Process for Hydrogen Production by Photosynthetic Bacteria,TQ116.2
  2. Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
  3. Control of Verticillium Wilt Disease of Cotton Plants with the Application of a Bio-Organic Fertilizer and Its Microbiologecal Mechanism in Rhizosphere,S144.1
  4. Effect of Simulating Soil Environment on Soil Bacterial Diversity by PCR-RFLP Analysis,S154.3
  5. Improvement in Electroporation Transformation Efficiency of Gene for Bacillus Subtilis with Trehalose,Q78
  6. Cloning and Expression of Organic Solvent Tolerant Lipase Gene from Staphylococcus Saprophyticus M36,Q78
  7. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  8. Screening and Isolation of One γ-PGA Producing Bacterium and Bilogical Effect of Bio-Organic Fertilizer,TQ922.1
  9. Antifungal Mechanism of Fengycin Against Rhizopus Stolonifer,TS201.3
  10. Studies on Microbial Contamination and Microbial Decontamination of Chemical Substances of Fresh Chicken,TS251.1
  11. Study on Bacterial and Fungi Community Diversity of Different Particle Size in Paddy Soils under Long-Term Fertilization Treatments in the Tai Lake Region, China,S154.3
  12. Effect of Organic Nutritiveadditives on Soil Micro-ecological Restoration and Its Mechanism.,S143
  13. Ecological Control of Tobacco Bacterial Wilt in Continous-cropping Field and the Microecological Mechanisms,S435.72
  14. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121
  15. Identification、Diversity and Phylogenetic Analysis of Antagonistic Bacillus Isolated from Different Chinese Areas,S476.1
  16. Insecticidal Activities of Recombinant CryIAc Protein from Bacillus Thuringiensis and Solid Fermentation Products from Aspergillus Terreus PNR2,S476.1
  17. Analysis of Gene Ycze in Bacillus and Biocontrol Efficacy of Genetically Engineered Bacillus Expressed Harpin on Rice Bacterial Leaf Blight,S476.1
  18. The Influence of Early Intervention of Vitamina Combined with Mycobacterium Bovis Bacillus Calmette-Guerin on Lung CD11C+ and Anti-OVA Special IgE Expression in Adult SD Mice Asthma Model,R562.25
  19. Isolation and Identification of Photosynthetic Bacteria and Preliminary Study of Proteinase Secreted from Rubrivivax Gelationsus,Q93
  20. Alleviating Effects of Bacillus Subtilis on the Cucumber under Salt Stress in Substrate Culture,S642.2

CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Peanut
© 2012 www.DissertationTopic.Net  Mobile