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The metalloproteins MT electrochemical behavior as well as the metal protein p53 and DNA interactions

Author: LiXue
Tutor: WangJianXiu
School: Central South University
Course: Analytical Chemistry
Keywords: Metallothionein Nafion- mercury film modified electrode p53 DNA Fluorescence
CLC: Q51
Type: Master's thesis
Year: 2010
Downloads: 58
Quote: 0
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Abstract


Metal protein is produced by a combination of amino acids and the metal ion. Metal ions as the metalloproteins important cofactor, assume the role of the center of the activity center or structure. Metallothionein transcription factor p53 tumor suppressor two important metalloproteins. The metallothionein (Metallothionein, MT) is a low molecular weight, high homocysteine ??levels, the combination of a large number of heavy metal ions in protein. MT is widely present in the human body, animals, plants, and microorganisms, it is involved in the balance of essential metal metabolism and heavy metal detoxification process. The study of the electrochemical behavior of MT elaborated oxidation reduction induced MT electron transfer with the metal release process as well as the role of environmental toxicology of great significance. MT electrochemical behavior of the research so far almost all of the mercury electrode, due to the strong binding force between MT and mercury MT in Cd and Zn ions may be replaced by mercury. Furthermore, the poor mechanical strength of the mercury electrode, and prone to contamination. The introduction of a Nafion membrane having selective permeation is expected to overcome the above drawbacks. The novel Nafion-Mercury membrane of modified the electrode (NCMFEs) two forms MT, Zn7-MT CdT-MT quantitative research. Respectively, -1.141 and -0.774 V at the redox peaks observed Zn7-MT Cd7-MT. The combined electrochemical and atomic absorption calculated each MT molecules of the electrode surface with the electrical activity of the metal - mercapto (M-Cysteine, M = Zn, Cd) side chains were 2.03 and 0.62. Indicating that an MT in the molecule, not all metals - cysteine ??side chains are involved in the oxidation-reduction reaction, only the distance the surface of the electrode closer to the group to be able to participate in the electron transfer process. The above work elaborated MT having an important role in the mechanism of the process in the metal transfer in Environmental Toxicology and intracellular. The tumor suppressor transcription factor p53 plays an important role in curbing the growth of tumor cells, DNA repair, and programmed cell apoptosis, hence the \The p53 protein is a combination of Zn ions, its biological function and DNA binding properties. p53 protein with the DNA binding region contains a zinc finger, zinc finger structure of zinc ion for the maintenance of the wild-type p53 (mutation type body) configuration, stability and activity of DNA bonded to shut important. Common detection of p53 DNA interactions gel mobility shift method, enzyme-linked immunosorbent assay (ELISA), and electrochemistry. Compared with the above-mentioned methods, fluorescence analysis is a simple, effective and sensitive means of detection. Can be embedded in the DNA helix by introducing a fluorescent probe to detect p53 DNA interactions. Further, by fluorescence resonance energy transfer (FRET), wherein the p53 as donor, DMACA modified DNA as the receptor, to indicate the interaction between p53 and DNA. The above method is sensitive and simple detection of p53 interaction with DNA process provides an important basis.

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CLC: > Biological Sciences > Biochemistry > Protein
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