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The Effect of L-carnitine and Exercise on Obese Mice FAT/CD36and CPT-1Enzyme in Skeletal Muscle

Author: Zhang
Tutor: ZhangYunZuo
School: Nanjing Institute of Physical Education
Course: Human Movement Science
Keywords: L-carnitine exercise CPT-1enzyme FAT/CD36
CLC: G804.2
Type: Master's thesis
Year: 2013
Downloads: 22
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Abstract


Objective: exogenous administration of L-carnitine and aerobic exercise onFAT/CD36and CPT-1enzyme in skeletal muscle of nutritional obese mice, soas to further explore the mechanism of L-carnitine and the role of exercise fromthe molecular level, and also provide more theoretical basis for the rational useof nutritional supplements to improve exercise capacity.Methods: nine mice were fed a normal diet (group C) among49C57BL/6mice, and40were established nutritional obese mice model (group H), whichwere fed high fat diet. After modeling, mice were randomly divided into obesequiet group (H), the obese L-carnitine group (HL), obese exercise group (HE),obesity carnitine combined with exercise group (HLE). HE and HLE wouldswim for5weeks. HL and HLE were administrated L-carnitine an hour beforeevery exercise. Weight of body of mice were monitored during the experiment,detecting the levels of free fatty acid (FFA), the content of CPT-1enzyme, andFAT/CD36expression amount in skeletal muscle at the end of the experiment.Results: After five weeks of exercise and L-carnitine supplement, the bodyweight of HE (15.17%) and HLE (9.64%) was significantly lower than group H(P <0.01)，the weight of HLE was lower than HL (p<0.05); FFA content ofskeletal muscle of HE (82.52%) and HLE (77.17%) was significantly lower thanH and HL groups (P <0.01), FFA content of HL (0.27%) was lower than H (P<0.05), FFA content of HE, HLE was lower than HL, respectively82.47%and77.11%(P≤0.01); the content of CPT-1enzyme in HL(22.9%), HE(22.6%), and HLE (47.92%) substantially significantly lower than group H (P=0.02, P<0.01); compared with group H, level of FAT/CD36expression of HE increasedby7.84%(P=0.05), level of FAT/CD36of HLE (25.88%) was lower thangroup H (P=0.03, P <0.05); content of CPT-1enzyme showed moderatepositive correlation with body weight; expression of FAT/CD36showedmoderate positive correlation with weight.Conclusion:1. There wass no obvious effect on L-carnitine supplementationalone on obese mice in controlling body weight, while there was significantintervention effect on exercise and the combination of L-carnitine sport andexercise in controlling weight.2. The intervention within each group of miceskeletal muscle FFA levels were significantly reduced, but the effect of exerciseand the combination of L-carnitine and sport promoting fatty acid oxidation andaccelerating fat metabolism was more obvious.3Exercise could increase theobese mouse skeletal muscle FAT/CD36protein expression, possibly by fattyacid transporter FAT/CD36content temporary increased for fatty acidtransshipment increasing.4Exercise, L-carnitine supplement and thecombination of L-carnitine and sport could reduce CPT-1enzyme levels.Exercise combined L-carnitine could interactional promote reducing theexpression of FAT/CD36. The content of CPT-1and FAT/CD36weremoderately positive correlated with body weight. Exercise and L-carnitineplayed a role through AMPK pathway.

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