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Effect of L-cysteine on the Bioleaching of Ni-Cu Sulphide and Chalcopyrite with Extremely Thermophilic Archaea
Author: ZhaoJianCun
Tutor: HeZhiGuo
School: Central South University
Course: Microbiology
Keywords: Bioleaching Nickel - copper sulphide ore Chalcopyrite L-cysteine DGGE
CLC: TF18
Type: Master's thesis
Year: 2010
Downloads: 45
Quote: 1
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Abstract
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The purpose of this paper is to further understand the extreme thermophilic archaea the different biological sulfide ore leaching, as well as L-cysteine ??(L-cysteine) leaching sulfide ore extreme thermophilic archaea. In this thesis, the L-cysteine ??on the sulfide ore the surface properties A.manzaensis and mixed extremely thermophilic archaea leaching of nickel-copper sulphide ore and chalcopyrite, while using DGGE-PCR analysis of mixed extremely thermophilic hot archaea leaching system variation of the microbial community in the process of sulfide ore. Zeta potential of nickel-copper sulphide ore before and after the combination with L-cysteine ??and the detection of infrared spectroscopy, found that L-cysteine ??chemical specific adsorption, and L-amino, carboxyl, and sulfhydryl nickel-copper sulphide ore cysteine ??amount of how many different variations cause the surface of the nickel-copper sulphide ore Zeta potential. A.manzaensis leaching of nickel-copper sulphide ore process, L-cysteine ??can accelerate A.manzaensis leaching of nickel-copper sulfide ore, metal leaching rate. Under the conditions of the nickel-copper sulfide ore concentration of 2%, concentration of 0.2g / L L-cysteine ??A.manzaensis leaching of nickel-copper sulfide ore have the greatest role in promoting, excess L-cysteine ??is not conducive A.manzaensis leaching nickel copper sulfide ore. L-cysteine ??can also improve A.manzaensis A.brierleyi, M. sedula, of S. metallicus nickel-copper sulphide ore and chalcopyrite leaching rate, and will affect the nickel-copper sulphide ore and chalcopyrite leaching system changes in the microbial community. Nickel-copper sulphide ore leaching system under conditions without the presence of L-cysteine ??in the early leaching system advantages flora Msedula, S. metallicus, late leaching system, A.manzaensis proportion somewhat elevated, system advantages flora to become S. metallicus and the A.manzaensis A. brierleyi only present in small amounts in the initial and final stages of leaching. Added L-cysteine ??in the nickel-copper sulphide ore leaching system early in the leaching system dominant flora A.manzaensis, M. sedula, S. metallicus A.brierleyi only present in small amounts; in dip The mine late advantage flora only A.manzaensis and S. metallicus M. sedula and A.brierleyi only present in small amounts. L-cysteine, in the process of the four kinds of bacteria mixed A.manzaensis, A.brierleyi M. sedula, S. metallicus leaching of chalcopyrite, play a leading role of S. metallicus and A.manzaensis . The M. sedula present in small amounts in the leaching process, improve only slightly in the latter part. L-cysteine ??added chalcopyrite bioleaching microbial system has remained relatively stable. Play a major role in the leaching process, which A.manzaensis from the fourth day A.manzaensis in proportion system has been maintained at about 50%, at the end of the leaching once accounted for 67.2%, M. sedula in The proportion in the system is maintained at between 16% -36%, while the proportion of S. metallicus system has been maintained at between 10% -25%.
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