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Isolation and Identification of Ferroplasma Thermophilum L1 and Its Application in Bioleaching of Chalcopyrite

Author: ZhangRuBing
Tutor: ZhouHongBo
School: Central South University
Course: Microbiology
Keywords: Ferroplasma thermophilum Bioleaching Chalcopyrite Real-time PCR Population dynamics
CLC: TF18
Type: Master's thesis
Year: 2008
Downloads: 97
Quote: 3
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Abstract


Bio-metallurgical technology is a method to extract metals from the mineral, especially suitable for processing lean ore and waste rock, and has a low cost, small investment, low energy consumption, little environmental pollution and other prominent advantages. In this study, an extremely acidophilic ferrous oxide moderate thermophilic archaea isolated and purified and identified as a new species of the genus Ferroplasma. Order to study the role of the archaea in sulfide ore leaching ancient physiological and biochemical characteristics of the bacteria studied thermophilic Thiobacillus the bacilli (to Acidithiobacillus caldus of), the addicted side Hook pylori (Leptospirillumferriphilum), eosinophilic curing tuberculosis (Sulfobacillus acidophilus) and different combinations of the archaea chalcopyrite leaching, and also investigated the variation of the parameters of the pH, the concentration of ferrous ions, trivalent iron ion concentration and the copper ion concentration in the leaching process, and application the Real-timePCR technology ScS microbial population changes discussed above strains mechanism of leaching of chalcopyrite. Isolated from the leaching solution of the leaching reactor a ferrous oxide archaea L1. Strain L1 morphological, physiological and biochemical characteristics. Spherical, can not exercise, no cell wall, the bacterial diameter 0.4 to 1.0. The optimum growth temperature of about 45 ° C, the optimum initial growth pH of 1.0, on behalf of approximately 6.65 h. Can exist simultaneously in the ferrous sulfate and yeast extract under the conditions of the growth of facultative nutritional This strain can not grow, but in the case of only ferrous sulfate or yeast powder. This strain is not available peptone, glucose, lactose and galactose and other organic matter as well as sulfur, sodium thiosulfate and other sulfur Growth reduced state. The strain L1 G-C content of the genomic DNA to 34.1mol%. Construct a phylogenetic tree based on 16S rRNA sequence homology, the results show that the strains to the ancient bacteria belonging to the genus Ferroplasma, with 99.0% sequence similarity with F.cupricumulans BH2 T . Strains L1 and F.cupricumulans BH2 T hybridization similarity of 46.3%. Based on the above experimental results show that the strain L1 should be representative of a new species of the genus Ferroplasma named F.thermophilum L1 (CCTCC, the AB207143 T ). Thermophilic Thiobacillus bacilli, to end Hook addicted pylori bacteria vulcanized acidophilus strain L1 four different combinations of chalcopyrite in shake flask leaching experiments. Of self-raising of facultative growth medium thermophilic bacteria exist can effectively promote the leaching rate of chalcopyrite leaching efficiency. The interaction between strains with different physiological characteristics (including iron-sulfur conversion and utilization of organic and inorganic) is conducive to promoting the leaching of chalcopyrite. In leaching system containing addicted to end Hook pylori, chalcopyrite leaching rate began to decline in 15-20 days after leaching and jarosite passivation film formed in the surface of the mineral leaching process. Slag leaching system X-ray diffraction analysis showed that the Fe 3 jarosite formation of lower concentration of the leaching solution, with respect to pH, the Fe 3 of The concentration is the the jarosite formation of the more important. Using real-time quantitative PCR analysis of bioleaching microbial populations in the early leaching the thermophilic sulfur tuberculosis bacilli and addicted to end Hook pylori accounted for a large proportion, but by the late leaching addicted to end Hook pylori population the proportion is significantly reduced. F.thermophilum occupy a small number of early in the leaching to leaching late occupy a large proportion in the population. The vulcanized acidophilus proportion of the population did not change much. The experimental results are also adapted to their physiological and biochemical characteristics.

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