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Influence of Immunity in Mice by Sexual Hormone

Author: WuLiHua
Tutor: HuSongHua
School: Zhejiang University
Course: Clinical Veterinary Medicine
Keywords: Immune Sex hormone levels Ginsenosides Spleen lymphocytes Cytokines Androgen receptor Immune response Female mice Females Serum mRNA Lymphocyte proliferation Expression levels Specific antibodies Estradiol Inhibitors Lymphocyte transformation Estrogenic activity Strengthen the immune Male mice
CLC: S852.4
Type: Master's thesis
Year: 2010
Downloads: 120
Quote: 0
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Abstract


Prevention of infectious diseases is the focal work on large-scaled farms, and vaccination is one of effective ways against infectious diseases. But sometimes animals failed to response to immunization and infectious diseases take place. Immune response is very complicated process and can be influenced by many factors. Sex hormone is one of them. In the present thesis, effects of exogenous estrogen and testosterone as well as endogenous sex hormones on the immune responses were studied.1、Effect of exogenous estrogens on the immune responses in female miceObjective To evaluate the patterns of immune responses to ovalbumin (OVA) of mice injected with estrodiol or ginsenosides Rbl and Rgl. Methods Seventy-two female ICR mice were randomly divided into nine groups with eight mice in each. The animals were subcutaneously immunized twice at 3 week intervals with saline or OVA (10μg) in saline or with OVA in saline containing 17β-estradiol benzoate (30μg) or ginsenoside Rbl (50μg) or Rgl (50μg). Treatment with the antiestrogens (ICI182,780 200μg, sc, daily) was initiated one week before immunization. For measurement of serum OVA-specific IgG and IgG subclasses, blood samples were collected from the orbital venous sinus 2 weeks after the primary immunization and the boost. Sera were isolated and kept at-80℃until use. Splenocytes were harvested at 2 weeks after the booster immunization for determination of lymphocyte proliferation and cytokines mRNA expression. Results The stimulation by 17β-estradiol benzoate or ginsenosides Rbl and Rgl significantly increased IgG response and serum OVA-specific IgG1 lever to OVA immunisation. Mice immunized with OVA plus ginsenoside Rbl and Rgl had higher splenocyte proliferative response to Con A, LPS and OVA than the mice injected with OVA alone with the highest response found in the group adjuvanted with Rgl (P<0.05). Ginsenosides Rbl and Rgl also significantly increased the expression of both Th2 cytokines (IL-4 and IL-10) and Thl cytokines (IFN-y and IL-12) mRNA as well as the expression of both T-bet and GATA-3 mRNA.All the immunomodulatory activity of estrodiol and ginsenosides were partly or completely blocked by the ICI182,780. Conclusions Estrogen has up-regulated Th2 immune response,and Ginsenosides Rb1 and Rg1 have up-regulated both Thl and Th2 immune responses.There are certain relationship between the immune response and estrogen receptor.2、Effect of exogenous androgen on the immune responses in male miceObjective To evaluate the patterns of immune responses to ovalbumin (OVA) of mice injected with testosterone propionate or ginsenosides Rbl and Rgl. Methods Seventy-two male ICR mice were randomly divided into nine groups with eight mice in each. The animals were subcutaneously immunized twice at 3 week intervals with saline or OVA (10μg) in saline or with OVA in saline containing testosterone propionate (50μg) or ginsenoside Rbl (50μg) or Rgl (50μg). Treatment with the antiandrogen (flutamide 300μg, sc, daily) was initiated 3 days before immunization For measurement of serum OVA-specific IgG and IgG subclasses, blood samples were collected from the orbital venous sinus 2 weeks after the primary immunization and the boost. Sera were isolated and kept at-80℃until use. Splenocytes were harvested at 2 weeks after the booster immunization for determination of lymphocyte proliferation and cytokines mRNA expression. Results The stimulation by testosterone propionate did not increase(without difference when flutamide was added) the levers of IgG and specific IgG1, IgG2a, IgG2b and IgG3, as well as T and B lymphocyte proliferation in response to Con A, LPS and OVA than when OVA was used alone (P>0.05). Mice immunized with OVA plus ginsenoside Rbl and Rgl had higher splenocyte proliferative response to Con A, LPS and OVA than the mice injected with OVA alone with the highest response found in the group adjuvanted with Rgl (P<0.05) Ginsenosides Rbl and Rgl also significantly increased the expression of both Th2 cytokines (IL-4 and IL-10) and Thl cytokines (IFN-y and IL-12) mRNA as well as the expression of both T-bet and GATA-3 mRNA. Conclusions Androgen can not up-regulate the immune responses of mice,and there are no relationship between the immune response up-regualted by ginsenosides and androgen recepor.3、Effects of the opposite sex on the immune responses in male and female miceObjective To evaluate the patterns of immune responses to ovalbumin (OVA) of mice with or without their opposite sex. Methods In experiment 1,30 female mice were randomly divided into 3 groups with 10 females in each and an additional male mouse was added in group 3. To prevent female mice from pregnancy, the male mouse was vasoligatured. In experiment 2, animals were grouped as in experiment 1 but females or male was replaced by males or female. The mice were subcutaneously immunized twice at 3 week intervals with saline (group 1) or OVA (10μg) in saline (groups 2 and 3). Blood samples were collected for measurement of OVA-specific IgG, estrodiol and testosterone, and splenocytes were prepared for determination of cytokines mRNA expression at 2 weeks after the booster immunization. Results The results showed that stimulation by the opposite sex significantly increased IgG response to OVA immunisation in both male and female mice, increased Th2 cytokines (IL-4 and IL-10) and decreased Th1 cytokines (IFN-γand IL-12) in female mice but decreased Th2 cytokines (IL-4 and IL-10) and increased Th1 cytokines (IFN-γand IL-12) in male mice. Conclusions Females have higher Th2 but lower Th1 immune responses,while males have lower Th2 but higher Th1 immune responses.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Immunology
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