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Atorvastatin on experimental autoimmune encephalomyelitis in rats and rat brain tissue LINGO-1 protein expression

Author: LvYiNing
Tutor: TanLiMing
School: Central South University
Course: Neurology
Keywords: Experimental Xing autoimmune encephalomyelitis Multiple sclerosis Atorvastatin LINGO-1
CLC: R965
Type: Master's thesis
Year: 2010
Downloads: 29
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Abstract


Objective To observe the atorvastatin pairs of experimental Xing autoimmune encephalomyelitis (experimental autoimmune encephalomyelitis, EAE) rats were the incidence situation, neurological function score, organizational pathological changes as well as the lateral ventricle the surrounding LINGO-1 expression the impact of, explore the atorvastatin right EAE model the impact of and the possible role of mechanism of. Methods in order to fresh guinea pig wholly-spinal cord homogenate (guinea pig spinal cord homogenate, GPSCH) plus pertussis vaccine of the original serous (pertussis vaccine, PV) and the completely Freund's adjuvant (complete freund's adjuvant, CFA) as antigen, immune inoculation female Wistar rats were , the establishment of EAE model. Will 78 female Wistar rats were randomly divided into 5 groups: normal control group six, EAE model group 18, EAE a small dose of atorvastatin group (2mg/kg-d) 18 只, EAE large doses of atorvastatin group (8mg/kg · d) 18 只, EAE prednisone group (5mg/kg-d) 18 只. Each drug intervention group rats were In the antigens after immunization the first 1 days began to, respectively, given corresponding drug intervention, once a day, until a were sacrificed the day before. The normal group In the at 15 days put to death. Rest of the group then according to sacrificed timing of your then randomly divided into a the 11th day, 15 days and 21 days three time points sub-groups, each sub-group of 6 only, and respectively, in its at corresponding time points were sacrificed. Observed in each group rats were incidence of situation, and conduct neurological function score. Fetch rat brain tissue lateral ventricle the surrounding tissue slices carried out detection: adopt hematoxylin Su - Yihong (hematoxylin-eosin, HE) staining, and in the observed under light microscope pathological changes and conduct inflammatory lesions counting; adopt Luxol fastness Blue - tar Purple nerve myelin staining France OK myelin staining, and in the observed under light microscope demyelination the situation; adopt immunohistochemical method, detect rats in each group lateral ventricle the surrounding tissue within the LINGO-1 protein expression the situation, and conduct positive cells were number count; adopt SPSS17.0 software on rat incidence rate, the incubation period, neurological function score, inflammatory number of lesions and LINGO-1 expression carry out correlation Xing statistically analyzed. Results 1. In each group rats were The clinical manifestations of: normal control group of rats in the experiment process without onset, nerve function Review Rated Bad 0 points; with the EAE group relatively, large doses atorvastatin group and the hormone group rats incidence of EAE's incubation period prolong (p lt; 0.05), incidence rate, neurological function score reduce the (p lt; 0.05); a small dose of atorvastatin group of although the latency was prolonged, the incidence rate, neurological function score reduced, but the difference was was no significant Xing (p gt ; 0.05). Large doses of atorvastatin group and the hormone group compare, rat of EAE incubation period, the incidence rate, neurological function score was no significant Xing difference in (p gt; 0.05). 2. Rats in each group the CNS HE and LFB staining pathological results: EAE group Yu the first 11 Timeless CNS has appeared inflammatory lesions, paras. 15 Timeless inflammatory lesions expand the scope of, increase in the number, the first 21 Timeless inflammatory lesions to reduce, extent alleviate the . With the same period EAE group compared to the, large doses atorvastatin group, hormone group with regard to CNS inflammatory number of lesions reduce the (p lt; 0.05); while the small-dose atorvastatin group was no significant Xing difference in (p gt; 0.05). Large doses of atorvastatin group and the over the same period hormone group comparative, CNS's inflammatory number of lesions was no significant Xing difference in (p gt; 0.05). 3. Rats in each group lateral ventricle the surrounding the white a qualitative LINGO-1 immunohistochemistry and image analysis results: Compared with the normal control group lateral ventricle the surrounding the white a qualitative LINGO-1 the expression level of comparative, EAE group the first 11 days, 15 days Asian group of the LINGO-1 the expression level of in both downward adjustment (p lt; 0.05), EAE group the first 21 days Asian group's LINGO-1 the expression level of raised the (p lt; 0.05). With the same period EAE group comparison: small dose of atorvastatin group LINGO-1 expression level of while no obvious change, difference was no significant (P gt; 0.05); the first 11 days, large doses atorvastatin group and the hormone group rats LINGO-1 expression level of changes in is not obvious, difference in was no significant Xing (P gt; 0.05); to fifth 15 days, large doses atorvastatin group and the hormone group rats LINGO-1 expression level of somewhat up-regulated the difference was significant Xing (P lt; 0.05), while the large dose atorvastatin group and the hormone between group was no significant difference Xing (P gt; 0.05); the first 21 days, hormone group rats LINGO-1 expression level of is low, differences in there are was significantly Xing (P lt; 0.05), while the large dose atorvastatin group LINGO-1 expression levels were significantly lower, the difference was significant Xing (P lt; 0.01). Conclusions a. Atorvastatin pairs of experimental Xing autoimmune encephalomyelitis in rats might has a protective effect, and in the a certain extent, showed a dose-related Xing. 2. Affect the the central nervous system's LINGO-1 protein in the expression level of, may be the atorvastatin exerts its protective role in one of the mechanisms.

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