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Objective: To detect runt -related transcription factor 2 (Runx2) in osteosarcoma tissue expression . Analysis of its expression and the incidence of osteosarcoma and clinical stage, pathological classification relationship. Explore Runx2 in osteosarcoma diagnosis and treatment possible role . Methods: Immunohistochemistry (SP method ) in 29 cases detected Runx2 osteosarcoma tissues and 15 cases of osteochondroma tissues , and analyze it in osteosarcoma tumor tissue and bone tissue and cartilage tissue osteosarcoma different stages , sub -type expression differences . Results : 1, Runx2 in osteosarcoma tissues , the positive rate was 96.6% , of which 17.2% weakly positive , moderately positive 41.4% , strong positive was 38.0 % in the control group, the positive rate of tissue osteochondroma 20.0% are weak expression , compared with control group , Runx2 expression in osteosarcoma tissues was significantly increased (P lt; 0.01). 2, Runx2 in osteosarcoma among different age groups , different genders , between different parts of the expression was no significant difference (P all gt; 0.05). 3 , osteosarcoma with Enneking Runx2 expression increased with increasing surgical staging , Ⅰ - Ⅱ A osteosarcoma tissues , Runx2 negative expression rate of 10.0% , 40.0%, weakly positive , moderately positive 40.0% , 10.0%, strongly positive , Ⅱ B-Ⅲ osteosarcoma tissues , no negative expression , weakly positive 5.3% , 42.1%, medium positive and strong positive 52.6% . Runx2 expression differences between the two groups was significantly (P lt; 0.01). 4, in the osteoblastic osteosarcoma , fibroblasts, bone sarcoma, osteosarcoma, cartilage, bone sarcoma three kinds of subtypes , Runx2 expression was not significantly different (P gt; 0.05). Conclusions : (1) Runx2 high expression of benign and malignant lesions of bone tissue and the occurrence of osteosarcoma . Tip Runx2 may be used as a secondary diagnosis of osteosarcoma indicator . (2) Runx2 Enneking surgical staging in the later osteosarcoma tissues higher , suggesting that Runx2 osteosarcoma may be used as a predictor of poor prognosis .
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