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Experimental Studies of the Sandwich-type Electrochemical Enzymes-based Lna-modified DNA Biosensor for Detection of Relative Fusion Genes in Acute Promyelocytic Leukemia

Author: SunZhouLiang
Tutor: LinXinHua
School: Fujian Medical
Course: Pharmaceutical Analysis
Keywords: Locked Nucleic Acid Electrochemical DNA biosensors Acute promyelocytic leukemia Mixed samples
CLC: R733.71
Type: Master's thesis
Year: 2010
Downloads: 41
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Abstract


Rapid, sensitive, miniaturized and easy to operate by the widespread concern in the new biosensors - electrochemical DNA biosensors is considered open up interdisciplinary electrochemical and molecular biology, genetic engineering, medicine has far-reaching significance and application value. Locked nucleic acid probe is a new probe technology in recent years with the development of genetic engineering techniques, has a strong DNA, RNA recognition ability and affinity, high thermal stability characteristics, genetic research has been applied to various areas as well as the diagnosis and therapy. In this study, for the first time to design a new type of electrochemical probes (locked nucleic acid), and then build a model of a \and examine the performance of the sensor specificity, stability: the locked nucleic acid probe technology with electrochemical enzyme-linked immunoassay technology combined to build a \detecting change in current before and after hybridization, as the indication signal. The results show that: the optimal hybridization conditions, the sensor can be in the 1.0 × 10 -13 to 1.0 × 10 -11 mol / L DNA concentration range of quantitative determination of synthetic PML / RARα fusion gene, and the concentration of the complementary strand with the sensor detection current signal into a good linear relationship between the detection limit is 7.4 × 10-14 mol / L. Completely complementary sequence in the hybridization solution with different wrong with the sequence can better distinguish synthetic hybrid system alone PML / RARα fusion gene qualitative and quantitative determination of ideal results. Second, the mismatched sequence of the different types of mixing as interfering background, the specificity and sensitivity of the \The results indicate that under optimum conditions, the electrochemical DNA biosensor measurement mixing interference system synthetic PML / RARα fusion gene of the linear range of 5.0 × 10 -9 ~ 5.0 × 10 -11 mol / L, to detect The limit is 9.6 × 10-12 mol / L. Shows that the sensor has strong molecular recognition ability under certain interference conditions, can completely complementary sequence in the hybridization solution hybridization, for clinical acute promyelocytic leukemia samples detection research has laid the foundation.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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