Dissertation > Excellent graduate degree dissertation topics show

The Empirical Study of Effect of Autophagy Inhibition on Endothelial Progenitor Cells

Author: ZhuRenDa
Tutor: LiXiaoQiang
School: Suzhou University
Course: General Surgery
Keywords: Endothelial Progenitor Cell Autophagy 3-methlyadenine Autophagy inhibition Flow cytometry
CLC: R329
Type: Master's thesis
Year: 2010
Downloads: 107
Quote: 1
Read: Download Dissertation

Abstract


Objective: To investigate the effect of proliferation、apoptosis and cell cycle of autophagy inhibition on endothelial progenitor cells.thereby to create a new method for the therapy of the deep venous thrombosis.Methods:1、Bone marrow-derived mononuclear cells were isolated from rat bone marrow by ficoll and cultured with EGM-2MV medium cultivation.observe the features and biological characteristics of EPCs. 2、Experimental design for the five groups, the control group and four concentrations of 3-methlyadenine (3-MA). 1.25mmol/l、2.5mmol/l、5mmol/l、10mmol/l. MTT method used to detect various concentrations (3-MA) proliferation of endothelial progenitor cells. 3、Flow cytometry(FCM) were used to detect cell apoptosis and cell cycle. 4、Monodansylcadaverine staining and Transmission Electron Microscope (TEM) were used to examine autophagy induced by 3-MA in endothelial progenitor cells.5、Western blot analysis were used to detect the LC3-II protein expression changes.6 SPSS 13.0software was used for analysis. mean expression by (±s). Single-factor analysis of variance used to compare the average of each group. (P <0.05) have statistically significant.(P﹥0.05) without statistical significance.Result:1.The rat bone marrow EPCs were islated successfully in vitro.2. 5mmol/l 3-MA promote proliferation of endothelial progenitor cells. compared with the control group was statistically significant (P <0.05). 10mmol/l 3-MA inhibit the proliferation of endothelial progenitor cells . Compared with the control group was statistically significant (P <0.05). The remaining two groups had no significant effect on proliferation of endothelial progenitor cells. 3. 10mmol/l 3-MA can promote apoptosis of endothelial progenitor cells(P <0.05). EPCs in the other groups have no significant difference in apoptosis rate. 5mmol/l and 10mmol/l 3-MA influence endothelial progenitor cell cycle distribution. 10mmol/l 3-MA can occur for S phase of endothelial progenitor cells increased (P <0.05). G2/M phase cells decreased (P <0.05).5mmol/l 3-MA can reduce the G0/G1 phase cells and increased S and G2/M phase cells(P <0.05). There were no significant differences in the other two groups on the cell cycle. 4. Autophagy inhibition was induced in EPCs at the concentration of 5mmol/l and 10mmol/l 3-MA as detected by both MDC staining and TEM. 5. 3-MA can reduce LC3-II protein expression of endothelial progenitor cells by detection of Western blot . 5mmol/l and 10mmol/l 3-MA significantly reduced protein expression of endothelial progenitor cells(P <0.05).Conclusion: 3-MA could inhibit endothelial progenitor cells in autophagy. A moderate inhibition of autophagy can promote the proliferation of endothelial progenitor cells. Excessive autophagy inhibition can inhibit the proliferation of endothelial progenitor cells. Redistribution of the cell cycle may be one of the mechanisms affecting cell proliferation. Moderate inhibition of autophagy did not significantly affect the apoptosis of endothelial progenitor cells. Excessive inhibition of autophagy may promote apoptosis of endothelial progenitor cells.

Related Dissertations

  1. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  2. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  3. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  4. Effect of Different Glucose Concentrations on Autophagy in Neonatal Rat Cardiomyocyte Culture,R542.2
  5. Apoptosis Mechanisms Research in Interfering TIGAR Gene and Epirubicin Treatmnet in Hepatic Carcinoma Cells,R735.7
  6. The Effect of Gefitinib in Radiosensitization Through Autophagy Regulation in Human Non-small-cell Lung Cancer A549 Cell Line,R734.2
  7. Amiloride Is Protective in PC12 Cells Via Autophagy Pathway,R329
  8. The Effects of ER Stress on Cell Toxicity and Htt Levels in the Stable PC12 Cells Expressing Htt586,R363
  9. Anti-tumor Effects of Lupeol at Different Regimens and Its Role in Immune Systems,R730.5
  10. Intervention and Mechanisms of E-64d on Developmental Seizure-induced Cognitive Deficit,R720.597
  11. Effects of Lysosomal Enzyme Inhibitor on Recurrent Neonatal Seizure Induced Acute and Long-term Gene Expression and Behavioral Changes,R720.597
  12. Study on the Autophagy Related Gene after Focal Cerebral Ischemia/reperfusion and the Effects of Artificial Synthetic E-selectin in Rats,R651.1
  13. Experimental Study the Role of Autophagy in Early Brain Injury after Subarachnoid Hemorrhage in Rats,R743.35
  14. The Effects of Autophagy on Vascular Smooth Muscle Cells Proliferation,R363
  15. Effects of siRNA Inhibit Class I PI3K on Humangastric Cancer SGC7901 Cells,R735.2
  16. Effects of Combined Therapy of 5-FU and Inhibitor of ClassⅢ　PI3K Human Gastric Cancer SGC7901 Cells,R735.2
  17. Effects of Mutant Huntingtin-552 on Glutamate Transporter GLT-1 in Astrocytes Are Reversed by Rapamycin,R742.2
  18. CD96 Expression on Hemopoietic Stem Cells in Acute Leukemia,R733.7
  19. Functional Analysis of Autophagy-related Genes MgATG11、MgATG13 and MgATG17 in Magnaporthe Oryzae,S435.111.41
  20. Effect of Calpain 2 and Autophagy Related Gene 7 on Non-alcoholic Fatty Liver Disease,R575.5
  21. The Effects of Autophagy on Cardiomyocytes Viability and Mitochondrial Membrane Potential in Sepsis,R459.7

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile