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Systematic study vitex (Vitex trifolia L.) and leaf disc If the chrysanthemum (Prenanthes macrophylla Franch.) The chemical composition of the two plants. Vitex (Vitex trifolia L.) plants (Verbenaceae), Verbenaceae Vitex (Vitex), about 250 species of this genus worldwide, mainly distributed in tropical and temperate regions, China has 14 kinds. Modern pharmacological studies have shown that, in the case of some plants, or a component has analgesic, anti-inflammatory, anti-bacterial, anti-tumor inhibit lymphocyte and hormonal regulation, antioxidant, molting, deworming and other effects. Vitex plant contains chemicals and the ecdysteroid ketones, terpenes, flavonoids and lignans compounds. Large leaf dish fruit Ju (Prenanthes macrophylla Franch.) Plate fruit daisy family Asteraceae (Compositae) are (Prenanthes) plants, about 40 species of this genus worldwide, mainly distributed in tropical and temperate regions of about 16 kinds. Which do medicinal cure carbuncle sores poison blood hemostatic effect. The chemical constituents currently few reports, which mainly contains triterpenes, sesquiterpenes, phytosterols. This thesis is a systematic study of the chemical composition of the two plants vitex (Vitex trifolia) and big-leaf dish fruit Ju (Prenanthes macrophylla), using conventional silica gel column chromatography (CC), dextran (Sephadex LH-20) which was purified column chromatography the microporous resin chromatography (MCI) and by preparative high performance liquid chromatography (HPLC) and other experimental means. Use of modern spectroscopic (UV, IR, EI-MS, HR-ESI-MS, 1H-NMR, 13C-NMR, DEPT, 1H-1HCOSY, HSQC, HMBC, ROESY, etc.) on the isolated compound of the structural identification, and the study of anti-tumor activity of some of these compounds. 31 compounds were identified, 28 of which (V1 ~ V28) of the compound obtained from Vitex isolated structure, respectively, triterpenes, fatty acids, flavonoids and derivatives of benzene, wherein the compound V28 for new natural products, the compound V3, and V4 , V5, V7, V8, V11, V12, V15, V16, V18, V19, V24, V25, for the first time from this genus were isolated; ethyl acetate extract layer was isolated 11 compounds from the the big leaf discs fruit daisy 10 (P1 ~ P10), which identified a phenylacetyl glycosides compounds, other were triterpenes, sesquiterpenes, flavonoids and organic acids, all compounds were isolated from this plant for the first time . In vitro antitumor studies have shown that the new compounds of the K-562, HL-60 and SMMC-7221 cell line cytotoxic activity of two sesquiterpene compounds have strong anti-tumor activity in vitro. In this paper, for the first time by high performance liquid chromatography to of sesquiterpene glycosides large leaf dish fruit Ju grass (P4) to determine the content, results showed that the P4 is linear in the range of 0.44 ~ 3.52μg, r = 0.999 7 of the Act simple, fast, good reproducibility, P4 content of as large leaf discs fruit Ju grass in measurement method. This research provides evidence of the chemical composition of plant classification, at the same time provide the chemical basis for development and utilization of natural medicine resources. Finally genus Vitex medicinal activity and chemical composition studies reviewed.
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