Dissertation > Excellent graduate degree dissertation topics show

Experimental Study on the Therapy of Umbilical Cord Mesenchymal Stem Cell Transplantation in MRL/lpr Mice

Author: XiaoYuCui
Tutor: WangJiBo
School: Qingdao University
Course: Internal Medicine
Keywords: umbilical cord mesenchymal stem cell transplantation Lupus erythematosus,systemic mice,inbred MRL/1pr
CLC: R593.241
Type: Master's thesis
Year: 2011
Downloads: 31
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE:To investigate the efficacy of umbilieal cord mesenehymal stem cells(MSCs) transplantation in the treatment of the MRI/lpr mice.METHODS:Thirthy two 16-weeks-old MRL/lpr female mice were divided into 4 groups:control group,CTX group, CTX+MSCs group, and MSCs group. The mice (usually at 4 to 5 months of age) with autoimmune diseases in CTX and MSC+CTX group were received intraperitoneal injections of CTX at a dose of 100 mg/kg body weight for 2 days. One day after the injection,1×106 MSCs were injected into the MSCs and MSCs+CTX groups via tail vein The control group were treated with 0.5mL normal saline as controls. Twenty-four hours proteinuria and body weight were assessed every four weeks. The levels of serum anti-dsDNA antibodies and IL-10、IL-17、IFN-γ、PDGF were measured by enzyme linked immunosorbent assay (ELISA). The histopathology changes of the UC-MSCs surface marks CD105 and CD44 in lung and spleen were RESULTS:The levels of IL-10、IL-17 in CTX+MSCs group was decreased than that in other groups, the difference between them was statistically significant.(P<0.05). The levels of IFN-y in CTX+MSCs group was incresased than that in other groups, the difference between them was statistically significant.(P<0.05).At the 32th weeks,the 24 hours proteinuria in CTX+MSCs group (4.22±1.53) mg/L and MSCs group (5.82±1.23) mg/L was decreased than that in control group (9.81±2.32)mg/L and CTX group (9.64±1.92) mg/L (P<0.01).The difference between CTX+MSCs group (2.63±0.92) and control group (4.83±0.72) of serum levels of anti ds-DNA antibodies was statistically significant(P<0.05). However, there was no significant difference in serum levels of ANA between the treatment and control groups. After MSCs transplantation, glomerular hardening treatment and inflammatory cells infiltrating degree relieved than that in the control group.⑤At the 32th weeks, the expression of MSCs surface marks CD 105 and CD44 in lung and spleen was negative.Conclusion:MSCs transplantation can reduce lupus activity of MRL/Ipr mice significantly and have marked therapeutic effect for lupus nephritis, It can also inhibit immune svstem bv reducing the levels of inflammation factors

Related Dissertations

  1. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  2. Ginsenoside Rg1 on human umbilical cord blood CD34 ~ hematopoietic stem cell proliferation and differentiation , and the impact of calcium-sensing receptor,R285.5
  3. Research of Biological Characteristics and Function of Umbilical Mesenchymal Stem Cells,R329
  4. Scaffolds Prepared with Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co 3-hydroxyhexanoate) for the Hepatic Differentiation of Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton’s Jelly,R329
  5. Quality Control of ABO Blood Group Typing for Neonatal Umbilical Cord Blood,R446.6
  6. Relationship of IL-17 and Th17 Cells to Systemic Lupus Erythematosus and Effect of Dexamethasone on PBMCs Secretion of IL-17 and Expression of Th17 Cells,R593.241
  7. Human Umbilical Cord Mesenchymal Stem Cells in Patients with Aplastic Anemia Hematopoietic Regulation of Negative Regulatory Factors,R556.5
  8. Study of Biological Features of CD3~+ CD56~+ NKT Like Cells in Peripheral Blood of Patientswith Systemic Lupus Erythematosus,R593.241
  9. CD34 ~ cells under different conditions to the endothelial cells in vitro,R329
  10. Preliminary Study on Angiogenesis in Ischemic Hindlimb of Diabetic Rat Model after Mesenchymal Stem Cell Implantation,R587.1
  11. Association of FCRL3 Expression Levels with Systemic Lupus Erythematosus,R593.241
  12. Dynamic Variation of Pregnant Women Serum Lipids、Oxidized-Ldi Concentration and Character of Umbilical Blood Lipids,R446.1
  13. Research on Senescence of Bone Marrow Mesenchymal Stem Cells in Patients with Systemic Lupus Erythematosus,R593.241
  14. 1、HBeAg Can up Regulate the Frequency of Regulatory T Cell in Cord Blood 2、Factors of Elevated ALT in CHB Patients Treated with Nucleos(t)ide Analogues for 48 Weeks,R714.2
  15. Preliminary Discussion of Law about Immune Reconstitution after Unrelated Cord Blood Transplantion and the Relationship Between CD3-16+56-NK Subsets Reconstruction and Clinical Efficacy,R733
  16. Effect of Mesenechymal Stem Cells on Epithelial-mesenchymal Transition with Human Colon Cancer Cells SW480 and Probable Mechanism in Vitro,R735.35
  17. Tetracycline pollutants in aquatic environments and human detection method in body burdens of PBDEs Research,X832
  18. The Study of Seeding Methods for Human Umbilical Cord Derived Mesenchymal Stem Cells Tissue Development in 3-Dimentional Matrix,R329
  19. The Characteristics of Umbilical Cord Blood T-cell Repertoire Based on Detection of T-cell Receptor Genes Rearrangement,R392
  20. Differentiation of Mesenchymal Stem Cells Derived from Human Umbilical Cord into Neuro-like Cells,R329
  21. Biological Activity and Gene Expression Changes of Long-term Cultured Human Umbilical Cord MSCs,R329

CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Lupus erythematosus > Systemic lupus erythematosus
© 2012 www.DissertationTopic.Net  Mobile