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To obtain proline through the cultivation of plant suspension cells, in this paper, Suaeda salsa was acted as a research object, and single factor test and orthogonal trial were used as research methods, ultimately, we might perform the inducement of Suaeda salsa callus, the establishment of suspension cell system, and the optimization of suspension cell culture medium and culture conditions. The main results were shown as follows:ⅰ. Different basic culture medium and plant hormone combinations were used to research the callus growth under light or dark culture conditions. As a result, medium①(MS+2,4-D 2mg/L+ 6-BA 1mg/L) in light condition were the most suitable for Suaeda salsa callus culturing, and the inducing rate of the callus got over 70% using cotyledon explants.ⅱ. The suspension cell system of Suaeda salsa was established, and the best inoculable period was between 5th and 10th days and the subculture period of the suspension cells was at 12th days.ⅲ. After optimizing cells suspension culture of Suaeda salsa, the proline content reaches 1074.50μg/mL, and the medium combinations and the culture conditions for suspension cells were:MS medium containing hormone 2,4-D 2mg/L +6-BA 1mg/L, inoculum size of 25%, adding sorbitol as a carbon, adding potassium nitrate a nitrogen, and 120r/min speed of shaker under natural light at 25℃.ⅳ. NaCl, as a kind of elicitor, was used to explore it’s influence on proline accumulation during the cell culture, the results indicated that the proline content was 2516.32μg/mL in the medium containing 2.0% NaCl(w/v) of 500mL shake flask, which was a highest content,2.8 times by the control groups.In this paper, it will provide us some preliminary parameters for proline accumulating that the relaxed callus are acquired using the inducement of cotyledons’ callus and the subculture of those callus and the cell suspension culture medium and culture conditions are optimized.
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