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Study of the Combination of Microfluidic Chip and Raman Tweezers

Author: AiMin
Tutor: LiuJunXian；YaoHuiZuo
School: Guangxi Normal University
Course: Theoretical Physics
Keywords: Raman tweezers microfluidic chip optical tweezers Raman spectroscopy automation
CLC: Q6-33
Type: Master's thesis
Year: 2009
Downloads: 56
Quote: 0
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Abstract


Raman tweezers is an optical technology coupled optical tweezers and Raman spectroscopy, also called the Raman spectroscopy combined with optical trap (RSOT) technique or the laser tweezers Raman spectroscopy (LTRS) system. A laser beam is introduced into an inverted microscope through a high numerical aperture objective to form an optical trap termed optical tweezers. The optical tweezers can trap a single particle in three dimensions nearly in the balance of the focus of laser beam. The same laser beam is used to excite Raman scattering of the trapped particle. The scattering light from the particle is collected by the objective and coupled into a spectrograph. With the analysis of the Raman spectra data, different particles or cells can been recognized and distinguished. It is one of the hot tools on single cell- molecular level in biology field. Microfluidic chips are molecular analytical tools for solving the complex analytical challenges associated with the field of biology. Last decade, microfluidic chips have been advanced sharply, which can be named micro-total analysis systems (μ-TAS), lab-on-a-chip or integrated chemistry lab and so forth. Under ideal condition,μ-TAS can intergrate very course of conventional chemical experiments in the laboratory in the same microdevice, which also is a recent technology in current science study. Microfluidic Raman tweezers combined these two techniques offers a new tool for manipulation and identification of single biological cells and microscopic particles automatically.This thesis includes six chapters. Chapter 1 is an introduction, including the history, theory, application and current study of Raman tweezers. The main contain is the theories of Raman spectroscopy and optical tweezers, and the application of their combination in biology. The second chapter is the study base of microfluidic chips, mainly containing basic character of microsize, the material and facture of microfluidic chips, and the facture, placement and cleanout of the chip of our laboratory. The third chapter is the device of Raman tweezes, including laser source, detector, coupled optical path, and so on. The fourth chapter is the capability test of Raman tweezers, including the experiments of rat single cells and polystyrene beads. The fifth chapter is the advance and integration of Raman tweezers, mainly introducing the integration and application of microfluidic Raman tweezers. The sixth chapter is the advance of next study. In this thesis, we designed and implemented an integrated automatic platform in Raman tweezers, also called microfluidic Raman tweezers, for simultaneous capture and analysis of the single particles automatically. This system makes it possible for the self-acting cycle of control, including delivery in the microchip, identification for capture by computer, and simultaneous saving and analyzing the Raman spectra of individual both biotic and abiotic particles. The microfluidic Raman tweezers system overcomes the unwanted contrived disturb and provides a promising automatic tool for identifying suspicious agents. These techniques include single channel microfluidic devices, LTRS, a computer controlled digital image processing and program links.The microfluidic Raman tweezers system has been applied for the study in cell biology at the single cell level. With the built microfluidic Raman tweezers system, we studied the performance of the system by single living cells, including red blood cells and white blood cells for Raman tweezers, and polystyrene beads for microfluidic Raman tweezers. We also use polystyrene beads to estimate the fluid velocity of the liquid. Theses studies show that the microfluidic Raman tweezers is feasible to provide automatically rapid and reliable diagnosis of particles.

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CLC: > Biological Sciences > Biophysics > Biophysical research and experimental > Biophysical techniques
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