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In this study, Yanbian cattle ear skin fibroblasts as donor cells , fluorescence ( UV ) explore the use of auxiliary to the core method of Yanbian cattle somatic cell cloning efficiency . Explore a looking for a simple, relatively no damage to the nuclear high efficiency to go nuclear , and thus lay the foundation for somatic cell nuclear transfer efficiency , and provide a theoretical basis for the industrial production . Test Results: 1 , 5μg/mLHoechst333420 min , 10 min , 20 min , 30 min , 40 min staining efficiency of Yanbian cattle oocytes . The results show that in the dyeing efficiency 20 min , 30 min , 40 min of staining was significantly higher than 0 min and 10 min . 2 , fluorescence - assisted enucleated oocyte enucleation rate and squeeze to remove nuclear law for comparison. In the the enucleated accuracy , the fluorescence - assisted enucleated method reached 100% enucleation rate , squeeze to the nuclear method was significantly lower than fluorescence - assisted enucleated law . 3 , more in the 10s , 15s, 20s, 25s , 30s the UV operating time of cleavage and blastocyst rate . The results showed that : on the cleavage rate of UV irradiation time 10s group and 15s group , 20s , 25S group of undifferentiated superior to UV irradiation time of 30s . 10s group on the blastocyst rate , 15s group and 20s group the highest , 25S group , followed by 30s group blastocyst rate minimum. 4 , the trials comparing fluorescence secondary to the core method and squeeze to remove nuclear restructuring embryo development rate . Parthenogenetic development group for the control group , results show that the five groups were no significant differences cleavage rate , blastocyst rate , the highest control group ( the parthenogenetic development group ) , 20 min staining to the core group , 30 min staining go nuclear group and 40 min staining to the core group had no significant difference , but significantly higher than the extrusion method to the core group . 5 , the experimental oocytes after dyeing after 5μg/mLHoechst33342 20 min , UV irradiation is less than 20s to operate nuclear law and intracytoplasmic nuclear injection method of oocyte cleavage and blastocyst rates compare with bet the impact . The results showed that : taken off the injection method is superior to the cytoplasm Note nuclear law .
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