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Use Gold Nanoparticles to Analyse and Detect Reduced Glutathione and Metal Ions

Author: XuWenJie
Tutor: LiZhengPing
School: Hebei University
Course: Analytical Chemistry
Keywords: glod nanoparticales reduced glutathione ultra-visible spectrophotometric resonance light scattering chemical sensor Hg(Ⅱ)
CLC: TB383.1
Type: Master's thesis
Year: 2008
Downloads: 42
Quote: 0
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Abstract


Gold nanoparticles has been widely used to determination biomolecules.In the first part of the paper, We utilize chemic and optical feature of gold nanoparticles to determination reduced glutathione. The interaction between GSH and gold nanoparticles was studied. Through the covalent combination with the-SH group and the electrostatic binding with the-NH3+ group of GSH, which results in the absorption peak of gold nanoparticles at 520nm move to the 640nm. On this basis, a new rapid and simple method using ultra-visible spectrophotometric to determining reduced GSH is established. At the optimal conditions, GSH can be selectively determined in the range of 0.01-0.20μg/ml(R=0.9988) with the detection limit of 3.0ng/mL(3σ).Most amino acids do not interfere with the determination.Chemical sensors are highly valuable in a variety of fields such as environmental chemistry, analytical chemistry, and bio-medicinal science. They provide accurate, on-line, and low-cost detection of toxic heavy metal ions with high selectivity and sensitivity. In the second part of the paper, a novel strategy for using gold nanoparticles capped with glutathione (GSH) for sensing heavy metals ions (Hg2+) was reported. Through the chelation between the GSH on the gold nanoparticles surface and heavy metals ions (Hg2+), the gold nanoparticles can be aggregated to form the net work structure, which results increase of the resonance light scattering (RLS) at 545 nm. Based on the study, a new rapid and simple method using resonance light scattering to determine heavy metal ions (Hg2+) was established. Under the optimal conditions, Hg2+ can be selectively determined in the range of 0.2×10-6-1.0×10-6 mol/L with the corresponding detection limit of 3.27×10-7 mol/L (3σ,0.5×10-6 mol/L).

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