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The Study of Molecularly Impinted Solid Phase Extraction-HPLC for the Determination of Quinolones

Author: LiuGuangYu
Tutor: SunHanWen
School: Hebei University
Course: Analytical Chemistry
Keywords: Molecular Imprinting Solid Phase Extraction High Performance Liquid Chromatography Quinolone Urine Serum Chicken
CLC: TQ460.72
Type: Master's thesis
Year: 2008
Downloads: 24
Quote: 0
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Abstract


Molecular Imprinting Technology (MIT) is an effective method to a specific molecular recognition sites introduced into the polymer matrix. Widely used because of its structure-activity scheduled specific recognition and extensive practical features. Molecularly imprinted polymer (MIP) as the solid-phase extraction packing molecular imprinting technique most promising development direction. Molecularly imprinted solid-phase extraction (MISPE) overcome the traditional solid-phase extraction selectivity defects, specific analysis of objects in complex samples or impurities selective extraction, thus greatly improving the precision and the accuracy of the analysis of the test, and reduce the the detection limit. In this paper, the preparation of molecular imprinted polymers for solid phase extraction - high performance liquid chromatography on a variety of quinolone drugs in body fluids and chicken separation and determination. The paper is divided into the following four parts: Chapter 1: Overview molecular imprinting and solid phase extraction technology knowledge, the introduction of the concepts and applications of molecularly imprinted solid phase extraction, and Molecular Imprinting Technology quinolones Summary. Chapter II: Building a simultaneous detection of human urine pipemidic acid, enoxacin, fleroxacin, lomefloxacin, pefloxacin, ciprofloxacin, enrofloxacin quinolones inverting high performance liquid chromatography. Adjusting the composition of the buffer solution and the proportion of the organic and water systems, to determine the composition of the mobile phase, to make a variety of quinolones good separation, the proposed method is simple, fast, and reliable. Chapter: methacrylate as functional monomer, ofloxacin template molecule, a novel molecular imprinted polymers were prepared, and their identifying characteristics, adsorption characteristics of solid-phase extraction conditions were discussed and optimization. The results showed that the imprinted polymer selective adsorption of structurally similar fluoroquinolones. Prepared molecularly imprinted polymer for solid phase extraction - high performance liquid chromatography separation of serum fluoroquinolones. Chapter 4: methacrylic acid as a functional monomer, enrofloxacin as template molecules in highly polar solvent methanol - water system were prepared by a new type of molecular imprinted polymers and their identifying characteristics, adsorption characteristics, chromatographic behavior were explored and optimized. The results showed that the imprinted polymer selective adsorption of structurally similar fluoroquinolones. Chicken samples with acetonitrile extraction and concentration over molecularly imprinted solid phase extraction column and eluted with acetonitrile cleaning and acetonitrile - trifluoroacetic acid (99:1, v / v), separated by high performance liquid chromatography. Prepared molecularly imprinted polymer for solid phase extraction chicken - high performance liquid chromatography separation and determination of four fluoroquinolones.

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CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > General issues > Product testing and analysis and identification > Drug analysis , drug identification
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