Dissertation > Excellent graduate degree dissertation topics show

Preliminary Study on Eperythrozoon Suis Subunit Vaccine

Author: LvZhou
Tutor: ZhangShouFa
School: Yanbian University
Course: Preventive Veterinary Medicine
Keywords: Eperythrozoon suis antigen subunit vaccine immunizing level
CLC: S858.28
Type: Master's thesis
Year: 2009
Downloads: 19
Quote: 1
Read: Download Dissertation

Abstract


Eperythrozoonosis is a zoonosis(anthropozoonosis) in many kinds of animals, which attached to erythrocytes,dissociated in plasma and marrow.Eperythrozoonosis of swine mainly causes fever,icteroanaemia and embryonic death,is mixed with other diease that took difficulty for prevention and cure,makes largely economic losses.In this study,to decollement eperythrozoon suis on base of extracorporal cultivanted eperythrozoon suis,it was splited to be eperythrozoon suis antigen by supersonic wave;antigen of E.suis were analyzed use SDS-PAGE and Western blotting.The specific antigen molecular weight was 58kDa and 29kDa.E.suis antigen was diluted kinds of different concentration by PBS;mice were immunized and then screening the best immunized concentration is 3mg/mL.The mice were immunized with eperythrozoon suis antigen which were combined with vash oil adjuvant,Freund’s adjuvant,alunitum adjuvant,alhydrogel adjuvant,At the same time we set up PBS control group;then immune response were observed by indirect ELISA.To compare immunizing effection about different adjuvant.The results showed that antibody level rised visiblely in a week;PBS control group and no adjuvant group have not change;vash oil adjuvant group and Freund’s adjuvantgroup’s antibody level higher than others groups;they were diversitied significantly with control group after secondary immunity a week.(p<0.05) Until deuto-week ad tertium vicem immunifaction,experimental group’s antibody level achieved peak then decreased gradually,the group of rash oil adjuvant and Freund’s adjuvant diversitied significantly with other groups(p<0.05),and they have significant significantly than control group.(p<0.01) that the group of vash oil adjuvant have the best immunity effection.We also detected ratio CD4~+ and CD8~+ of immunized mice to appraise subunit vaccine cellular immunity level,The results showed that the group of vash oil adjuvant;Freund’s adjuvant;alhydrogel adjuvant and alunitum adjuvant achieve the best immunized response,lymphocyte percent higher than signifcant significantly(p <0.01),the group of vash oil adjuvant and Freund’s adjuvant higher than no adjuvant group(p<0.05),alhydrogel adjuvant group’s lymphocyte percent higher than alunitum adjuvant group a little.To determined immunoprotection effect about subunit vaccine,we carried out eliminating toxic material text on partly immunity mice after about a week ad tertium vicem immunifaction.To detected information of infecting E.suis on immunized mice by PCR.The results showed that experimental group was detected E.suis on 37d after infection,and control group was detected E.suis on 3d after infection.This study supplied some theory foundation for the aspect of investigated vaccine and it also established some contribution for diagnosing and preventing.

Related Dissertations

  1. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  2. Development of Subunit Vaccine of Streptococcus Suis Type 2 and Actinobacillus Pleuropneumoniae,S858.28
  3. Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against Lps Antigen of B. Melitensis,R392
  4. Identification of New Protective Antigens of Streptococcus Equi SSP. Zooepidemicus,S855.11
  5. The Cloing、Expression and Initial Assessment of Immunoprection of Thioredoxin Glutathione Reductase of Schistosomiasis Japonicum,R392
  6. The Clinical Value of Detection of Serum TPS, CEA, CYFRA21-1, NSE for Diagnosis in Patients with Lung Cancer,R734.2
  7. DNA Prime/Protein Boost of Japanse Edcephalitis Virus NS1 Protein to Improve Immune Response in Mice,R392
  8. Diagnosis Study on Schistosomiasis Japonicum in Domestic Animals with Recombinant Antigen pGEX-BSjGCP-BSj23 as Diagnosis Antigen,S855.9
  9. Prepration of Artificial Antigen and Establishment of Protein Chip for Determination of Fumonisins B1,S816.17
  10. Construction of ActA/PlcB Null Mutant of Listeria Monocytogenes,S852.61
  11. Effect of Esculentoside a on Apoptosis and Expression of PCNA、Caspase-3、Fas and FasL in Renal Tissue of BXSB Mice,R285.5
  12. Heterologous antigens to the biological characteristics of cancellous bone research,R318.08
  13. Fucosyl transferase Ⅶ the expression promoting proliferation of A431 cells,R730.2
  14. Brain myocarditis virus VP1 fragment gene of stability of the , prokaryotic expression and the Applied Research,S852.65
  15. In-vitro Expression of Proliferating Cell Nuclear Antigen and Production of Polyclonal Antibody against PCNA from Prorocentrum Donghaiense,Q946
  16. Protection Against Tuberculosis by Vaccination with a Recombinant Bcg Strain Over-expressing HspX Protein,R392
  17. Domoic acid monoclonal antibody anti- competitive ELISA for the direct and kit assembly,X835
  18. Establishment of Double Antibody-Sandwich ELISA for Detecting E.ovis,S855.99
  19. Development of the Illict Drug Detection Kit Based on Small Molecule Microarray,S859.84
  20. Research of Actinobacillus Pleuropneumoniae Bacterial Ghost Loading of Pasteurella Multocida Outer Membrane Protein (Omp) H DNA Vaccine,S858.28
  21. The Research on Preparation and Properties of Antigen-extracted Heterologous Bone Scaffold Material,R318.08

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
© 2012 www.DissertationTopic.Net  Mobile